performed functional assays on T1AM, SEP-363856, RO-6889450, fenoldopam and A77636 with the help of P.C., S.G. and Heli Wang. K.W., S.G. and C.W. contributed to the G-protein-dissociation assay. W.Z. performed radioligand-binding assays. X.Z. contributed to the purification of...
Protein synthesisis the process oftranslatingmRNA to functional peptides. Translation begins with the formation of the 80S initiation complex, composed of mRNA, charged methionyl-tRNA, and both the 40S and 60S ribosomal subunits (Fig. 4.2). This cascade of events begins with the binding ofeukaryot...
For example, we can retain or even enhance the natural antibacterial, biomineralization and antifouling activity of proteins involved in film formation, and the properties can also be expanded through the physical blending or chemical grafting of additional functional blocks on the surface of the film...
To test the influence of lysine acetylation on the enzymatic activity, the Pta protein was first treated in vivo by acetylase (At2), and then a certain amount of the mixture (equivalent to 0.003 μM Pta protein in the final reaction solution) was used for the following Pta activity assay....
Finally, C8 included 10 C. S. DAWSON ET AL. Figure 3. Functional enrichment analyses of the significantly enriched and exclusive C. albicans EV proteins. The online tool FungiFun2 (elbe.hki-jena.de/fungifun) was used to identify enriched biological process (BP), cellular component (CC), ...
Activated agarose (Pegarose) beads have uniform size in the range of 50~150 microns. These activated beads have multiple functional groups that can be used to attached various bio-ligands covalently to the bead surface. Covalently linked ligands offer high stability and are suitable for various bi...
Thousands of genetic variants have been associated with complex traits through genome-wide association studies. However, the functional variants or mechanistic consequences remain elusive. Intermediate traits such as gene expression or protein levels are
7c). Like wild-type β-arrestin2 recruitment assay, Galanin among the ligands induced the maximal response in recruitment of three β-arrestin2–FlasH constructs. We then tested whether Galr2 activation produces an intramolecular Nluc–β-arrestin2–FlasH BRET signal upon recruitment to untagged ...
As potential TFs, all SPL7-like proteins contain a functional bipartite NLS overlapping ZF2 within the SBP-domain [11],[12],[26]. However, our cellular and biochemical approaches confirmed the anchoring of SPL7 to the microsomal fraction, most likely to the ER-membrane, through a C-...
As potential TFs, all SPL7-like proteins contain a functional bipartite NLS overlapping ZF2 within the SBP-domain [11],[12],[26]. However, our cellular and biochemical approaches confirmed the anchoring of SPL7 to the microsomal fraction, most likely to the ER-membrane, through a C-...