y Charoen, C. 1976. Automated modified Lowry method for protein analysis of milks. J. Food Sci., 41: 1219-1221Huang, Y. W., R. T. Marshall, M. E. Anderson, and C. Charoen. 1976. Automated modified Lowry method for protein analysis of milk. J. Food Sci. 41:1219-1221....
The Lowry method ( Chapter 1 in vol. 1 of this series) has been widely used, but is susceptible to interference from ... JBW Hammond,NJ Kruger - 《Methods Mol Biol》 被引量: 1396发表: 1988年 Lowry protein determination by automated flow injection analysis for bovine serum albumin and ...
The protein concentrations of an LDL preparation and a bovine brain homogenate were first determined by the modified Lowry method using BSA as a standard. Assays were then performed using the CBQCA Protein Quantitation Kit on samples containing from...
Accurately quantifying total protein concentration is a key step in most experiments and workflows involving isolation, separation, and analysis of proteins. We offer a variety of assay reagents, kits, and standards for protein quantitation by fluorescent or colorimetric detection with fluorometers, spect...
The stopped-flow time difference analysis technique (a novel and sensitive technique, involving a sort of end-point assay using the stopped-flow method) was applied to the modified biuret method (micro-biuret method) for protein determination. Protein concentration was easily determined from a reacti...
especially the widely used Lowry method, for several reasons. First, the Bradford protein assay is much easier to use. It requires one reagent and 5 minutes to perform as compared to the three reagents and 30–40 minutes typical for the Lowry assay. Second, because the absorbance of the dye...
Analysis of Protein by Spectrophotometric and Computer Colour Based Intensity Method from Stem of Pea (Pisum sativum) at Different Stages Afsheen MS, Memon AN, Memon MS, Ansari AW and Arain BA. Analysis of Protein by Spectrophotometric and Computer Colour Based Intensity Method from Stem of Pea...
Post Tags: #BCA#Bicinchoninic Acid#Bradford#Folin-Lowry#Kjeldahl#protein quantification#UV absorption More 'Protein Expression and Analysis' articles Gain Control: The Tet-On/Tet-Off Inducible Expression System ByRobert Williams Entering the Digital Age of Ultrasensitive Enzyme-linked Immunosorbent Assays...
Amino acid analysis is the only protein analysis method where interfering substances do not affect the results. Although there is potential for improvement in regards to the hydrolysis method, we recommend that this method should be the preferred for food protein determination. 展开 关键词:...
The protein concentration of the supernatants was analyzed by the method of Lowry et al. [22]. The sample aliquots (protein concentration of 2.0 mg/mL) containing 25 μg protein were used for SDS-PAGE. Molecular weights of the gel samples were determined on a 4% acrylamide stacking gel ...