J Biophys Method . 1983Noble R C. The colorimetric estimation of protein by the Lowry technique using a lquid scintillation counter[J].Journal of Biophys Method,1983,(03):223.Noble RC and Shand JH.The colorimetric estimation of protein by the Lowry technique using a liquid scintillation ...
Interference caused by Tris buffer in the method of Lowry et al. (NAR 21, 4458) can be overcome by incorporating the same amount of Tris in the standards used. Distortion at <0.15 mM Tris was within tolerable limits. Sodium phosphate buffer, 40 mM, did not interfere. An automated Lowry ...
Protein concentration was estimated by Lowry et al.64 method, using bovine serum albumin (BSA) as standard. Splenic lysate (60 µg/well) was resolved in 15% SDS-PAGE and transferred onto the nitrocellulose membrane in the transfer buffer (25 mM Tris, 193 mM glycine, 20% methanol, pH 8....
The Bradford Method For Protein Quantitation Nicholas J. Kruger Pages 17-24 Ultrafast Protein Determinations Using Microwave Enhancement Robert E. Akins, Rocky S. Tuan Pages 25-34 The Nitric Acid Method for Protein Estimation in Biological Samples ...
Estimation of the pore size of the large-conductance mechanosensitive ion channel of Escherichia coli. Biophys J 73, 1925–31 (1997). 52. Kowalczyk, S. W., Grosberg, A. Y., Rabin, Y. & Dekker, C. Modeling the conductance and DNA blockade of solid-state nanopores. Nanotechnology 22, ...
Fig. 3. Estimation of protein mass per cell using two biochemical approaches and the proteomic ruler method. A, the histone proteomic ruler concept. The mass of cellular DNA is approximately equal to the protein mass of histones. Relating the histone MS signal to the total MS signal therefore...
Standard amino acid analysis (AAA) by OPA/FMOC method for PM2.5 air filter samples Standard hydrolysis This method was adopted from the manufacturer’s protocol (AdvanceBio Amino Acid Analysis, Agilent Technologies). A filter sample (1 out of 16 pieces) was placed in a suitably sized Schlenk ...
Evolutionary engineering was accomplished by using the natural yeast mutation rate and mutagenesis procedures. One strain (P5) outcompeted the others under both experimental conditions and was able to impose after 200 generations. The evolved strains showed improved growth and low-temperature fermentation...
Heterotrimeric G proteins are fundamental signaling proteins composed of three subunits, Gα and a Gβγ dimer. The role of Gα as a molecular switch is critical for transmitting and amplifying intracellular signaling cascades initiated by an activated G
(2008) reported a protein yield of 219 mg/grice bran by subW as determined by the Lowry method, while the composition of the feedstock showed a mass percent of protein of 18.56%. In literature, it is reported that the Cu2+ ion present in the reagent is overly sensitive to some amino ...