This method of protein estimation is also known as the Lowry method because in 1951, Lowry et al. used this principle for the estimation of proteins in the biological samples. In this method, protein reacts with Folin phenol reagent to produce a blue color. The absorbance of this blue ...
Protein concentration was estimated by Lowry et al.64 method, using bovine serum albumin (BSA) as standard. Splenic lysate (60 µg/well) was resolved in 15% SDS-PAGE and transferred onto the nitrocellulose membrane in the transfer buffer (25 mM Tris, 193 mM glycine, 20% methanol, pH 8....
The interaction of proteins with specific DNA sites is a principal step in the control of many cellular processes. Over the past 20 years, our knowledge of protein-DNA interactions has grown geometrically. This growth has been facilitated by the availabi
Fig. 3. Estimation of protein mass per cell using two biochemical approaches and the proteomic ruler method. A, the histone proteomic ruler concept. The mass of cellular DNA is approximately equal to the protein mass of histones. Relating the histone MS signal to the total MS signal therefore...
The protein concentration determined by AAAA(Phe) was normalized to 1 mg/mL for all tested protein samples. The LOD was estimated to a protein concentration (BSA equivalent) of 7 mg/L for AAAA(Phe) and 0.8 mg/L for AAAA(Tyr) and is lower compared to the former method based on the ...
Estimation of globular protein secondary structure from circular dichroism. Biochemistry 20, 33–37 (1981). Article CAS Google Scholar Manavalan, P. & Johnson, W.C. Jr. Variable selection method improves the prediction of protein secondary structure from circular dichroism spectra. Anal. Biochem....
(2008) reported a protein yield of 219 mg/grice bran by subW as determined by the Lowry method, while the composition of the feedstock showed a mass percent of protein of 18.56%. In literature, it is reported that the Cu2+ ion present in the reagent is overly sensitive to some amino ...
Aurangabad, from the present estimation. The experimental fishes were collected in the live condition in the local fresh water sources brought to the laboratory. The fishes of similar size were selected. Lowry's `C' method is used to calculate the protein value in muscle sample of selected ...
There are many methods used to determine the amount of protein in a sample; the most important one is the Kjeldahl (Lynch and Barbano, 1999), Warburg (UV absorption method), Coomassie-blue (Bradford method), biuret, and Folin–Lowry methods (Sapan et al., 1999). However, MS can also ...
Reference serum and rabbit serum were diluted and pre-adsorbed for cross-reacting antibodies by treatment with pneumococcal CWPS and either 22F PS or 25PS. Human monoclonal anti-polysaccharide antibodies (Pamlico Biopharma, USA) were used for total IgG estimation. Bio-Plex 200 (Bio-Rad). ...