Here, it was aimed to estimate the protein content by Lowry's method at different wavelengths by UV-visible spectrophotometric analysis. Using Bovine Serum Albumin (BSA) particularly as a standard protein estimation of proteins is done because of its low price, high purity and ready availability....
Lowry法是双缩脲法和福林酚法的结合与发展,其原理是:蛋白质溶液用碱性铜溶液处理,形成铜-蛋白质的络合盐,再加入酚试剂后,除使肽链中酪氨酸、色氨酸和半胱氨酸等显色外,还使双缩脲法中...阅读全文 Bradford法蛋白定量(Bradford-Protein-Assay-) Bradford Assay is a rapid and accurate method commonly used t...
Pretreatment of protein samples containing mono- or dithiol reagents with N-ethylmaleimide significantly reduces interference during subsequent protein determinations by the Lowry method. When the Lowry procedure as modified by M. A. K. Markwell et al. ( Anal. Biochem. 87, 206) is utilized, the...
Interference caused by Tris buffer in the method of Lowry et al. (NAR 21, 4458) can be overcome by incorporating the same amount of Tris in the standards used. Distortion at <0.15 mM Tris was within tolerable limits. Sodium phosphate buffer, 40 mM, did not interfere. An automated Lowry ...
The presence of nonionic and cationic detergents interfered in the Lowry method of protein estimation by causing precipitate formation. The addition of 0.5% sodium dodecylsulphate in the alkali reagent prevented this precipitation without affecting colour development, and allowed the method ...
J Biophys Method . 1983Noble R C. The colorimetric estimation of protein by the Lowry technique using a lquid scintillation counter[J].Journal of Biophys Method,1983,(03):223.Noble RC and Shand JH.The colorimetric estimation of protein by the Lowry technique using a liquid scintillation ...
Modified micro lowry method utilized the principle of lowry method of protein estimation with certain modifications for estimation of myocardial tissue soluble collagen. During the test sample preparation, test solution shacked vigorously after addition of Folin- Ciocaltue reagent and further incubated ...
- 1985 () Citation Context ...tilled water and stored at 4C until determining the total protein amounts. Supernatants were collected to determine the protein amount of each sample using a 750 nm wavelength using the Lowry method =-=[15]-=-. Initial Attachment Assay Cryopreserved bone...