Waterborg JH,Matthews HR.The Lowry method for protein quantitation. Methods in Molecular Biology . 1994J.H. Waterborg, H.R. Matthews, The Lowry method for protein quantitation, in: J.M. Walker (Ed.), The Protein Protocols Handbook, Humana press, New Jersey, 1996, pp. 7-9....
Protein Protocols Handbook, The Volume 0 || The Lowry Method for Protein Quantitation The most accurate method of determining protein concentration is probably acid hydrolysis followed by amino acid analysis. Most other methods are sensitive... Walker,M John 被引量: 0发表: 2002年 The Bradford Met...
Protein Determination by UV Absorption Alastair Aitken, Michèle P. Learmonth Pages 3-6 The Lowry Method for Protein Quantitation Jakob H. Waterborg Pages 7-10 The Bicinchoninic Acid (BCA) Assay for Protein Quantitation John M. Walker Pages 11-15 ...
Lowry MethodNatural Rubber Latex Proteins ProteinQuantitationProteins from natura lrubber latex (NRL) can cause local and systemic reactions in people who are allergic to it. Reducing the total protein in NRL products to minimize the allergenic potential requires methods of accurately estimating the ...
J.H. Waterborg The Lowry method for protein quantitation J.M. Walker (Ed.), The Protein Protocols Handbook, Humana Press, Totowa, NJ (2002), pp. 7-9, 10.1385/1-59259-169-8:7 Google Scholar [21] J. Folch, M. Lees, G.H. Sloane Stanley A simple method for the isolation and puri...
To validate the instrument, the protein content of the platelet aggregate was estimated by the Lowry method (Lowry et al., 1951). Aggregating agents used were ADP (doses (μ mol), 1.0, 0.1, 0.01, 0.005, 0.0025 and 0.001), Thrombin (doses (units) 0.25, 0.20, 0.15, 0.10 and 0.05) ...
The rhMANF concentration in brain supernatant was normalized to the total protein concentration of the sample determined with the Lowry method (DC Protein Assay, Bio-rad Laboratories, Hercules, CA, USA). Evans blue extravasation The integrity of blood–brain barrier (BBB) was evaluated with Evans...
Moreover, the assay can be suitable for quantitation of samples containing agents which commonly interfere with the routinely used detection techniques. The assay is a valuable addition to the Bradford and the Lowry techniques. Used in combination with an antigen-specific ELISA it gives a reliable ...
A protein determination method which involves the binding of Coomassie Brilliant Blue G-250 to protein is described. The binding of the dye to protein causes a shift in the absorption maximum of the dye from 465 to 595 nm, and it is the increase in absorption at 595 nm which is monitored...
The cells were fractionated, and the protein concentration was determined by the Lowry method. Approximately 100 μg of protein from each fraction were blotted onto Whatman 3 MM paper filters and allowed to dry. The dried filters were immerged in 50 mL of 5% (w/v) TCA and boiled for 2 ...