The presence of nonionic and cationic detergents interfered in the Lowry method of protein estimation by causing precipitate formation. The addition of 0.5% sodium dodecylsulphate in the alkali reagent prevented this precipitation without affecting colour development, and allowed the method ...
Lowry法是双缩脲法和福林酚法的结合与发展,其原理是:蛋白质溶液用碱性铜溶液处理,形成铜-蛋白质的络合盐,再加入酚试剂后,除使肽链中酪氨酸、色氨酸和半胱氨酸等显色外,还使双缩脲法中...阅读全文 Bradford法蛋白定量(Bradford-Protein-Assay-) Bradford Assay is a rapid and accurate method commonly used t...
Interference caused by Tris buffer in the method of Lowry et al. (NAR 21, 4458) can be overcome by incorporating the same amount of Tris in the standards used. Distortion at <0.15 mM Tris was within tolerable limits. Sodium phosphate buffer, 40 mM, did not interfere. An automated Lowry ...
Noble R C. The colorimetric estimation of protein by the Lowry technique using a lquid scintillation counter[J].Journal of Biophys Method,1983,(03):223.Noble RC and Shand JH.The colorimetric estimation of protein by the Lowry technique using a liquid scintillation counter.Journal of Biochemical ...
Modified micro lowry method utilized the principle of lowry method of protein estimation with certain modifications for estimation of myocardial tissue soluble collagen. During the test sample preparation, test solution shacked vigorously after addition of Folin- Ciocaltue reagent and further incubated ...
Wahl R, Geissler W, Maasch HJ 1985 Comparison of classical Lowry, modified Lowry and a dye-binding assay for the estimation of protein in allergen extracts and influence of different parameters on the modified Lowry assay. Biol Chem Hoppe Seyler 366:979 -984...