Efficiency of Real-Time PCR What is multiplexing? Taq Talk Video Series In the following instances, you will need to calculate the concentration of primers or probes in solution: You have synthesized your own primers or probes for the first t...
[Na+] calculation:[Na+] = Monovalent ion concentration +4 x free Mg2+.3. Primer Annealing Temperature: The primer melting temperature is the estimate of the DNA-DNA hybrid stability and critical in determining the annealing temperature. Too high Ta will produce insufficient primer-template ...
Volume is not a factor in the calculation Hi Norman, This calculator takes a mass* of nucleotides* and strand length. It returns the total number of copies of the strands present in that amount of material. Concentration* depends on your sample. if you have DNA* at a concentration of ...
[Na+] = Monovalent ion concentration + 4 × (Free Mg2+) 1/2 (all in molarity) The default value = 382.84 mM Temperature for Free Energy Calculation This is used to calculate the Gibbs free energy (ΔG) in the formula: ΔG = ΔH - T ΔS. The default value = ...
DNA-binding dyes can be classified as either nonsaturating or saturating. Saturating dyes — for example, SYBR®Green — inhibit PCR reactions when used above a certain (saturation) concentration. Nonsaturating dyes — for example, EvaGreen — do not inhibit PCR and can be used at a suf...
For the PCR amplification to be successful, the primers must bind to opposite strands of the DNA template so that both strands of the target are copied with each cycle. They are added to the reaction in molar excess (usually at a final concentration of 200 to 900 nM) because with each ...
formamide_conc: Concentration of formamide (mol/l) annealing_temp_c: Actual annealing temperature of the PCR reaction in (C) temp_c: Simulation temperature for dG (Celsius) max_nn_length: Maximum length for nearest-neighbor calcs tm_method: Tm calculation method (breslauer or santalucia) salt...
Performing Digital PCR Hands-On Each digital PCR platform has its own hands-on protocol. In this item, we have chosen to describe protocols used on… Copy Number Variation Calculation for your PCR Experiment Copy Number Variation (CNV) detection is a critical tool for estimating the variation...
Impact of concentration of universal primer on singlet PCR.Wentao XuZhifang ZhaiKunlun HuangNan ZhangYanfang YuanYing ShangYunbo Luo
PCR Primer Design Selective amplification of nucleic acid molecules, that are initially present in minute quantities, provides a powerful tool for analyzing nucleic acids (Saiki et al., 1985; Mullis et al., 1987). The polymerase chain reaction is an enzymatic reaction, which follows relatively sim...