Introduction: Multiple myeloma (MM) is a plasma cell malignancy and natural killer (NK) cells have demonstrated anti-MM activity in vitro. Anti-myeloma effect of NK cell against MM cells may be due to the interaction between killer cell immunoglobulin-like receptors (KIR) against different human...
3.1Primary cell cultures Primary cell cultureis defined as theex vivoculture of cells freshly obtained from a tissue. The main advantages of primary cells are their faithfultranscriptomicandproteomicprofile and closer proximity to physiological function and response. Some drawbacks are their requirements ...
After co-culture of activated primary NK cells with luciferase reporter target cell lines and the addition of dilutions of ADCC and isotype control antibodies, we are able to show target cell specific and dose-dependent killing effects. The luciferase reporter provides great convenience in measuring...
Tumor/T Cell Co-culture Assay T Cells (Total, CD4+, CD8+) Activation and Proliferation Assays Th Subsets Differentiation (Th1/2/17/Treg) Assays Treg Suppressive Assay B Cell Activation Assay NK Cell Cytotoxicity Assay M1/M2 Macrophage Polarization Assay Macrophage/T Cell Co-culture Assay Pha...
Numerous human and murine primary cell cultures, including fibroblasts [1], glial cells [2], astrocytes [3], and immune cells (e.g. NK cells) [4]. Culturing and reprogramming of human and murine embryonic cells [5] and pluripotent stem cells [6]. ...
Finally, ER compartmentalization was monitored in distinct PBMC subsets, including CD3−CD56+natural killer (NK) cells, CD3+CD56−T cells, CD14−CD19+MHC II+B cells, CD14−CD19−MHC II+DCs and CD14+MHC II+monocytes. First cell subsets were stained and then the TPT assay was perfor...
Region E1A of highly oncogenic adenovirus 12 in transformed cells protects against NK but not LAK cytolysis - ScienceDirect The sensitivity of a library of adenovirus-transformed rat cell lines to lysis with highly enriched populations of rat NK cells and LAK cells activated in ... DJ Kenyon,...
Cytotoxicity of HBD3 for dendritic cells, normal human epidermal keratinocytes, hTERT keratinocytes, and primary oral gingival epithelial keratinocytes in cell culture conditions - ScienceDirectHuman beta-defensin 3 (HBD3) is a prominent ... NL A,CLF B,AMB B,... - 《Toxicology Letters》 被引...
Adult tissues set the scene for a continuous battle between cells, where a comparison of cellular fitness results in the elimination of weaker “loser” cells. This phenomenon, named cell competition, is beneficial for tissue integrity and homeostasis. I
Dong-Hee Lee & Ha Won Kim & College of Medicine, University of South Alabama, Mobile, Alabama, USA Stephen W. Schaffer Department of Developmental Neurobiology, NY State Institute for Basic Research in Developmental Disabilities, New York, USA Eunkyue Park ...