We further found that only high-dose (8 Gy) but not low-dose (2 Gy) irradiation in one fraction could significantly enhanced in vivo anti-HCC activity of CXCR2-armed CAR-NK92 cells. Irradiation with 8 Gy significantly up-regulated the expression of NK cell-activating ligands on HCC cells...
NK92 or primary sorted NK cells (after 72 hours of prestimulation with 1000 U/mL IL-2 alone) were placed (3 × 105) in tissue culture–treated Transwells of 6-well plates that had previously been incubated with 50 μg/mL of the recombinant CH-296 fibronectin fragment (Takara Shuzo, Ot...
Asiaticoside pretreatment significantly restored the cytotoxicity of NK92 cells and aeNK cells from health donors against various tumor cell lines, including K562 myelogenous leukemia cells, OVCAR8 and A2780 ovarian cancer cells, which had been impaired by TGF-β treatment (Fig. 1C). Similar ...
Reprogramming TME cells via gene editing. Gene editing is mainly used to reprogram CD8+T cells, CD4+T cells, NK cells, TAMs, and TAFs. ① The proliferation of CD8+T cells is promoted by editing cytokine receptors on the CD8+T-cell surface (above). The function of exhausted CD8+T cells...
In 2023, Yao et al. co-cultured PDOs, CAFs, and NK92 or CAR-NK92 cells in a ratio of 1:2:5, and elucidated a novel mechanism underlying CAF-mediated suppression of NK cell function within the TME, suggesting a potential NK cell-mediated immunotherapeutic strategy, utilizing iron chelator...
cells was upregulated after co-culture with NK-92MI cells.PD-L1 expression was knocked down,thereby inhibiting the proliferation and promoting apoptosis of HCC cells,and inhibited by berberine that blocked the secretion of interferon gamma(IFN-g),thereby enhancing the anti-tumor effect of NK92-...
The formation of an immunological synapse (IS) is essential for natural killer (NK) cells to eliminate target cells. Despite an advanced understanding of the characteristics of the IS and its formation processes, the mechanisms that regulate its stability via the cytoskeleton are unclear. Here, we...
hematopoietic stem cell transplant and inin vitroculture systems9,10,11. Freshly isolated CD56brightNK cells can become CD56dimNK cells in culture9, namely in response to FGFR1-mediated signalling, however this is poorly understood in the context of a signal that shapes this response in lymphoid...
ADCC activity of PF-06747143 and m15-IgG1 parent antibody was evaluated in JVM-13 CLL tumor cell line, in presence of the NK-92 FcγRIIIA 158V (NK92 158V) cell line as effector cells (Conkwest). Antibodies were incubated for 4 h, with tumor cells and effector cells (1:10 ratio)...
4C), nectin-2 tetramers bound to each DNAM-expressing cell line with the exception of NK92DNAM-1 Y113A cells (Fig. 5A). Given that NK92 cells preferentially kill via NKG2D, we performed the killing assays in the presence of the NKG2D-blocking antibody 1D11. Here, expression of WT ...