Live imaging of coculture of NK92 cells (green border line) pretreated with or withoqut LIMKi3 and U87MG cells (white border line). Supplementary Video 8 Live imaging of coculture of NK92 cells expressing Lifeact-GFP (green) and U87MG cells (white border line) with or without NEP1-40...
cells was upregulated after co-culture with NK-92MI cells.PD-L1 expression was knocked down,thereby inhibiting the proliferation and promoting apoptosis of HCC cells,and inhibited by berberine that blocked the secretion of interferon gamma(IFN-g),thereby enhancing the anti-tumor effect of NK92-...
We further found that only high-dose (8 Gy) but not low-dose (2 Gy) irradiation in one fraction could significantly enhanced in vivo anti-HCC activity of CXCR2-armed CAR-NK92 cells. Irradiation with 8 Gy significantly up-regulated the expression of NK cell-activating ligands on HCC cells...
injection of NK92 cells expressing NK-CAR12 or T-CAR3 at an E:T ratio of 2:1 (Supplementary Fig. 8D). For their identification, REH cells and CD19 KO REH cells were labeled with CellTrace CFSE and FarRed, respectively. Subsequent analysis of peritoneal exudates by flow cytometry ...
Asiaticoside pretreatment significantly restored the cytotoxicity of NK92 cells and aeNK cells from health donors against various tumor cell lines, including K562 myelogenous leukemia cells, OVCAR8 and A2780 ovarian cancer cells, which had been impaired by TGF-β treatment (Fig. 1C). Similar ...
NK92 or primary sorted NK cells (after 72 hours of prestimulation with 1000 U/mL IL-2 alone) were placed (3 × 105) in tissue culture–treated Transwells of 6-well plates that had previously been incubated with 50 μg/mL of the recombinant CH-296 fibronectin fragment (Takara Shuzo, Ot...
G., Characterization of a human cell line (NK92) with phenotypical and functional characteristics of activated natural killer cells, Leukemia, 1994, 8: 652. 8. Jaffe, E. A., Nachman, R. L., Becker, C. G. et al., Culture of human endothelial cells derived from umbilical veins, J....
The toxic effect of NK cells can be enhanced by promoting their toxic effects or restoring the toxic effects of inhibitory cells. NK92 cells are an NK cell line with an indefinite proliferative ability that are widely used in clinical trials [206,207]. However, they exhibit lower toxicity to...
(CAR‑NK) cells for cancer therapy: current state, challenges and emerging therapeutic advances Pin Yao1,2, Ya‑Guang Liu3, Gang Huang3,4, Liangchun Hao5 and Runan Wang5* Abstract Immunotherapy has transformed the landscape of cancer treatment, with ...
Natural killer (NK) cells are innate lymphocytes that recognize and clear infected and transformed cells. The importance of NK cells in tumor surveillance underlies the development of NK cell therapy as cancer treatment. The NK-92 cell line has been successfully modified to express high-affinity ...