用于测定mab的亲和力的优选方法可见于:harlow等,《抗体:实验室手册(antibodies:alaboratorymanual)》,coldspringharborlaboratorypress,coldspringharbor,n.y.,1988;coligan等编,《免疫学实验室指南(currentprotocolsinimmunology)》,greenepublishingassoc.andwileyinterscience,n.y.,(1992,1993);和muller,《酶学方法(meth....
The STEMdiff™ NK Cell Kit protocol first uses animal component-free STEMdiff™ Hematopoietic - EB reagents for the generation of embryoid bodies (EBs) from hPSCs which are then further differentiated into CD34+cells. The kit includes all the EB reagents necessary for this step: ...
For further information regarding the culture and expansion of human NK cells using ImmunoCultTM NK Cell Expansion Kit, refer to the Product Information Sheet (Document #10000013013). Protocol Preparation of Complete ImmunoCult™ NK Cell Expansion Medium Preparation of ImmunoCult™ NK...
Figure 1 protocol of human NK cell expansion 2. 只需要30ml外周血即可开始实验。 3. 效应细胞比例高。经该Kit培养外周血单核细胞14天,效应细胞(NK和NKT细胞)可达到80%,NK细胞表面marker表达增高。 Figure2 Selective proliferation of NK cellsderived from PBMC ...
(2). The main NK cell population of the lymph node (CD56CD16) expresses low levels of NKp46 in resting cells, but expression is up-regulated by IL-2. NKp46 is a type I transmembrane protein with two extracellular Ig-like domains followed by a short stalk region, a transmembrane ...
Figure 1 protocol of human NK cell expansion 2. 只需要30ml外周血即可开始实验。 3. 效应细胞比例高。经该Kit培养外周血单核细胞14天,效应细胞(NK和NKT细胞)可达到80%,NK细胞表面marker表达增高。 Figure2 Selective proliferation of NK cellsderived from PBMC ...
In this report, we optimized a protocol to obtain NKAE cells by using four different culture growth media (RPMI, SCGM, TexMACs and NKMACs), two different NK cell sources: PBMC or CD45RA+cells and two distinct irradiated aAPC (K562mbIL15 or K562mbIL21). We determined that TexMACs was ...
Poster: NK cell expansion using scale up bioreactors for cell therapy Flyer: High-yield NK cell culture for allogeneic cell therapy applications Product Information User Guide: CTS NK‑Xpander Medium A streamlined protocol for generating iNK cells from PSC spheroids 常见问题解答 (FAQ) We recommend...
The detailed Magnetic Labeling and Enrichment Protocol follows. In summary, the Biotinylated Human NK Cell Enrichment Cocktail simultaneously stains erythrocytes, platelets, and most leukocytes except the NK cells. After washing away excess antibody, BD IMag™ Streptavidin Particles Plus ...
Purification Protein A or G purified from hybridoma culture supernatant Immunogen Murine myeloma cell line NSO-derived recombinant mouse NKp46-Fc chimera Glu22-Asn255Accession # Q8C567 Formulation Supplied in a saline solution containing BSA and Sodium Azide. Label Phycoerythrin (Excitation= 488 ...