Subculturing: Protocol:NK-92MI细胞Cultures can be maintained by centrifuging cells and resuspending cell pellet in fresh medium at 2 - 3 X 105 viable cells/mL. Centrifugation and full replacement of culture medium may be performed for the first subcultures. Cultures can then be maintained by ...
Acute GVHD in patients receiving IL-15/4-1BBL activated NK cells following T-cell–depleted stem cell transplantation Figure 1. Protocol treatment and NK expansion schema. (A) Treatment schema. Asterisk denotes time point that was delayed to days (D) 15 and 24 to meet Food and Drug Admin...
The protocol involves cultures initiated at 2.5 × 105 cells/mL in 25 mL in 1 L Vuelife culture bags, with addition of fresh media plus IL2 every 3 days to maintain an optimal density of NK-92 cells for expansion. Daily disruption of cell aggregates enhances NK-92 cells ...
The protocol involves cultures initiated at 2.5×105 cells/mL in 25mL in 1L Vuelife culture bags, with addition of fresh media plus IL2 every 3 days to maintain an optimal density of NK-92 cells for expansion. Daily disruption of cell aggregates enhances NK-92 cells expansion and viability ...
survival and cytotoxicity of Robo1-CAR-NK92 cells and to optimize the culture protocol.Methods Robo1-CAR-NK92 cells were constructed by lentivirus transfection.The Robo1 car positive cells were sorted,expanded and detected by flow cytometry.The levels of Robo1 expression in SH-SY5Y and U8...
Protocol Basic information [Components] 2 mL Anti-Biotin Microbead Particles, cell culture grade, corresponding to 4× 10⁸ Microbead Particles; Microbead Particles conjugated to monoclonal anti-biotin antibodies. 0.4 mL CD335 (NKp46)-Biotin, human – functional grade (100 µg/mL). ...
This study addresses manufacturing-related issues and aimed to develop a GMP-compliant protocol for the generation of NK-92/5.28.z therapeutic doses starting from a well-characterized GMP-compliant master cell bank. Commercially available GMP-grade culture media and supplements (fresh frozen plasma, ...
Protocol Basic information [Components] 2 mL Anti-Biotin Microbead Particles, cell culture grade, corresponding to 4× 10⁸ Microbead Particles; Microbead Particles conjugated to monoclonal anti-biotin antibodies. 0.4 mL CD335 (NKp46)-Biotin, human – functional grade (100 µg/mL). ...
Following this step, the infection protocol was repeated. Starting from day 1 after the second infection, transduced NK92 cells were purified using a FACS Aria II cell sorter (BD Biosciences, San Jose, CA, USA) based on expression of a GFP marker on the cell surface encoded by the vector...