The protocol involves cultures initiated at 2.5 × 105 cells/mL in 25 mL in 1 L Vuelife culture bags, with addition of fresh media plus IL2 every 3 days to maintain an optimal density of NK-92 cells for expansion. Daily disruption of cell aggregates enhances NK-92 cells ...
The protocol involves cultures initiated at 2.5 × 105 cells/mL in 25 mL in 1 L Vuelife culture bags, with addition of fresh media plus IL2 every 3 days to maintain an optimal density of NK-92 cells for expansion. Daily disruption of cell aggregates enhances NK-92 cells expansion and ...
This study addresses manufacturing-related issues and aimed to develop a GMP-compliant protocol for the generation of NK-92/5.28.z therapeutic doses starting from a well-characterized GMP-compliant master cell bank. Commercially available GMP-grade culture media and supplements (fresh frozen plasma, ...
survival and cytotoxicity of Robo1-CAR-NK92 cells and to optimize the culture protocol.Methods Robo1-CAR-NK92 cells were constructed by lentivirus transfection.The Robo1 car positive cells were sorted,expanded and detected by flow cytometry.The levels of Robo1 expression in SH-SY5Y and U8...