Provided are a culture vessel for NK cells and a method for culture of NK cells, which can provide high NK cell culture efficiency. The present invention has been completed by confirming that high NK cell culture efficiency can be achieved by a method of culturing a biological sample ...
摘要附图 同族专利 序号申请号 专利名称 状态 1 PCT/KR2020/003943 ADDITIVE COMPOSITION FOR NK CELL CULTURE MEDIUM, CULTURE METHOD FOR NK CELL BY USING SAME ADDITIVE COMPOSITION, AND COSMETICS COMPOSITION OBTAINED THEREBY FOR IMPROVING SKIN PROBLEMS PCT未进入指定国(指定期满)关于...
Carefully count cells by trypan blue exclusion method using Neubauer cell count chamber. • Centrifuge cells at 4 °C for 7 min at 300 × g. • Resuspend melanoma cells at a density of 0.75–1 × 105 cells/mL (see Note 2) in complete medium. • In a 6-well cell culture plate...
本发明公开了一种NK细胞体外培养方法,属于人类细胞的培养. The present invention discloses a method of NK cells in vitro, are cultured human cells. 本发明用PBS稀释的赫赛汀与用PBS稀释人免疫球蛋白,合并后均匀铺满培养瓶底,过夜;另取外周血行密度梯度离心,吸取单个核细胞,加入无血清培养基,调整细胞浓度为...
Objective To investigate the optimal culture time of the healthy population's NK cells.Methods Extracting the mononuclear cells in peripheral blood 15ml of 10 healthy individual with conventional mononuclear cell extraction method,and the mononuclear cells were put in the cultivation hole containing SCGM...
Culture method for large-scale preparation of clinical treatment level dermal multipotent stem cells for cell transplantation The invention discloses a culture, screening and amplification technology of clinical treatment level dermal multipotent stem cells. These bottlenecks are ... 朱宁文,王凌仪 被引量...
According to the method for in-vitro amplification of the NK cells, two antibodies CD3McAb and CD226McAb are simultaneously coated, so the cell factor synthesis and ADCC effect are promoted, and killing toxicity of the NK cells is remarkably improved; the activation and amplification on the NK ...
Generation of allogeneic CAR-NKT cells from hematopoietic stem and progenitor cells using a clinically guided culture method Article Open access 14 May 2024 Automated and closed clinical-grade manufacturing protocol produces potent NK cells against neuroblastoma cells and AML blasts Article Open access...
NK 细胞(natural killer cell,NK)可直接杀伤肿瘤和病毒感 染的靶细胞,对宿主自身正常细胞无杀伤作用。细胞因子 IL 2 及 IL 15 可调节其杀伤及分泌功能。DC 细胞(dendritic cell,DC)是 机体内最强的抗原提呈细胞,可表达共刺激分子,分泌细胞因子,诱导 1 初始免疫应答。NK 细胞通过直接作用或生物信号间的协同...
公开/公告日期: Apr 25, 2012 发明人: E.阿里西 被引量: 3 摘要: A method of large scale expansion and simultaneous activation of NK cells and NK- like T cells in a closed cell culture system is presented, wherein the expanded cells exhibit increased cytotoxicity.收藏...