RA Zimbres,PPBD Oliveira - 《Electronic Notes in Theoretical Computer Science》 被引量: 7发表: 2009年 Approaching to Immigrant Families – The Scope of Transgenerational and Culture Shock Model In this study, we have referred to the educational model of culture shock, and the transgenerational mod...
Using the enzymatic explant method, culture medium containing A83-01 further reduced the confluence time of the cells and increased A-eMSCs enrichment during the primary endometrial cell isolation stage. Furthermore, A83-01 enhanced the proliferation and maintained the differentiation potential of A-...
Define primary interest. primary interest synonyms, primary interest pronunciation, primary interest translation, English dictionary definition of primary interest. Principal, although not exclusive, interest and responsibility for accomplishment of a gi
Cell culture is a valuable tool used for growing and maintaining cells in a controlled ex vivo environment1. Primary cell cultures are a type of short-term cell culture where the cells being maintained are the same types that came directly from the tissue, resulting in a diverse mix of cell...
Cells were detached with 0.05% trypsin EDTA and subcultured, and expansion culture by subculturing was continued until the number of cells required for the test was obtained. Both cell lines were passaged twice after thawing, and the cells were spread onto 12-well tissue culture-treated plates...
Approximately 500 thousand cells were frozen in Recovery™ Cell Culture Freezing Medium (Thermofisher #12,648,010) and stored at -80 °C. An aliquot of 50 thousand cells was then used for ATAC-seq library preparation following the Omni-ATAC protocol as released by the Kaersten lab [61,62...
Presents the basic steps necessary for culturing primary human cellsIncludes blank pages at the end of each chapter for readers to jot down important notes -->Keywords: Cell Culture; Primary Human Cells; Laboratory Manual; Tissues; Organs; Techniques; Protocols; Biosafety; Stem Cells; --> ...
Cross, "The Russian Primary Chronicle," Harvard Studies and Notes in Philology and Literature 12 (1930): 75-320. Sergei Alekseevich Bugoslavskii, The Textology of Old Rus'/Tekstologiia drevnei Rusi Part 1 treats the works of 1831-36 in the light of what McGuire identifies as an enduring...
by the absence of the nuclear lamina protein lamin B128. The defective nuclear lamina in senescent cells is also evident by the depletion of the high-mobility group box 1 (HMGB1) protein in the nucleus, which is one of the most abundant non-histone proteins in the mammalian cell nucleus. ...
Efficient, facile gene modification of cells has become an indispensable part of modern molecular biology. For the majority of cell lines and several primary populations, such modifications can be readily performed through a variety of methods. However,