PBA was determined by the total immunoglobulin A, G, and M concentrations in the culture supernatants as measured by micro-enzyme-linked immunosorbent assay. F. nucleatum, at concentrations between 1 and 10 micrograms/ml, stimulated optimal PBA in monocyte-depleted cultures, whereas the pokeweed ...
Polyclonal B-cell activation in cultures was evaluated by enumeration of cells secreting immunoglobulin (Ig) and/or by quantitation of Ig released into culture supernates. Cord blood T cells and PBL after activation with concanavalin A or by prolonged incubation were used as sources of suppressor ...
This cytokine plays an important role in hematopoiesis (formation of blood cells), spermatogenesis, and melanogenesis. SCF may be used along with other cytokines to culture HSCs and hematopoietic progenitors. The expansion of these cells ex-vivo (outside the body) would allow advances in bone-...
( 1976 ) Induction of autoantibodies to red blood cells by polyclonal B-cell activators . Nature 263 , 60 .Hammarstïom, L., Smith, E., Primi, D., and Möller, G., 1976, Induction of autoantibody to red blood cells by polyclonal B-cell activators, Nature ( London ) 263 :60. ...
Now imagine that, instead of collecting the pooled sera, we isolate all of the immune cells from the animal and prepare them for cell culture in a multiwell plate. By diluting the mixture of cells so that each well receives either no cells or one cell, we can produce a single clone of...
Untreated THP-1 and THP-1 (treated with 80 nM PMA overnight, replaced the culture medium with 100 ng/mL LPS for 6 hours with addition of 300 ng/mL Brefeldin A for the last 3 hours) cells were subjected to SDS PAGE followed by western blot with (Product # 17590-1-AP) (TNF Alpha ...
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Mouse peritoneal cells were used as feeder cells (103/well). ELISA The reactivity of hybridoma culture supernatants was assessed by ELISA. Polyclonal antibodies to Tx-synthase in 50 mmol/L NaHCO3 were adsorbed to wells (1 μg) of microtiter plates during 1 hour at room temperature. Residual ...
cultureparticipation motivationphysical activityLYMPHOCYTES can be activated in vitro by a variety of so-called polyclonal stimulants or mitogens (reviewed in refs 1, 2 and 3). These responses characteristically involve a larger proportion of cells than observed with antigen as the eliciting ligand ...
Polyclonal antibodies (pAb) represent a collection of antibodies from different B cells that recognize multiple epitopes on the same target antigen (the part of an antigen that is recognized by the antibody) with a wide range of affinities. ...