This study delineates the culture conditions and cellular requirements for pokeweed mitogen (PWM)-induced polyclonal B-lymphocyte activation of rabbit Peyer's patch cells for immunoglobulin secretion. This system was optimal with a cell density of 3 脳 10 5 cells/ml and a culture duration of 7 ...
Two hybridomas were developed by fusing heteromyelomas to Epstein-Barr virus immortalized B cells obtained from beekeepers. The cloned hybridomas were stable and secreted up to 40 mg/L of antibody into the culture supernatant. Phospholipase A2 specificity of the human monoclonal antibodies was ...
Using differential centrifugation, size fractionation, and inhibition assays, it has been possible to map Mycoplasma to the exosome or vesicle fraction purified from culture supernatant (CSN). Mycoplasma fractions were shown to induce proliferation of B and not T cells. The B cell response was ...
Polyclonal antibodies (pAb) represent a collection of antibodies from different B cells that recognize multiple epitopes on the same target antigen (the part of an antigen that is recognized by the antibody) with a wide range of affinities. ...
cell culture media 内含物 0.1% sodium azide 保存条件 Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. 运输条件 Wet ice RRID AB_2244103 产品详细信息 PA1-29036 detects CCL4 from mouse, rat samples. ...
d-e, Linear regression for the number of barcode mutations over the time of culture (days) for ApcMin/+ normal organoids (d) and neoplastic organoids (e), respectively. f, The estimated number of founding progenitors (Np) for each polyp from Apc68 (left) or Apc72 (right). Cells ...
cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs).The GTP...
pET22b-ΦC31 expressed abundant fusion protein in YTG culture medium after being induced by IPTG 0.2 mmol/L for 30 hours.The specific polyclonal antibody against ΦC31 integrase was obtained from the sera of the His-ΦC31 integrase fusion protein immunized rabbits.Furthermore,this kind of protein...
F1 cells that had been positively selected in the SMLR with stimulator cells from 1 parental strain demonstrated a markedly enhanced proliferative response to antigen in association with the parental strain used in the SMLR selection culture. The observed genetic restriction on the antigen responses of...
d-e, Linear regression for the number of barcode mutations over the time of culture (days) for ApcMin/+ normal organoids (d) and neoplastic organoids (e), respectively. f, The estimated number of founding progenitors (Np) for each polyp from Apc68 (left) or Apc72 (right). Cells ...