Journal of NeuroimmunologyStangel M, Bernard D (2003) Polyclonal IgM influence oligodendrocyte precursor cells in mixed glial cell cultures: implications for remyelination. J Neuroimmunol 138: 25-30.Stangel M, Bernard D. Polyclonal IgM influence oligodendrocyte precursor cells in mixed glial cell ...
Two hybridomas were developed by fusing heteromyelomas to Epstein-Barr virus immortalized B cells obtained from beekeepers. The cloned hybridomas were stable and secreted up to 40 mg/L of antibody into the culture supernatant. Phospholipase A2 specificity of the human monoclonal antibodies was ...
a, FACS sorting of immune and epithelial neoplastic cells using the cell-surface markers, CD45 and EpCAM, respectively. Vertical and horizontal lines indicate the threshold for positive cells. b, The mutation burden for sorted immune cells and neoplastic cells, where a filter step for the tumor...
Now imagine that, instead of collecting the pooled sera, we isolate all of the immune cells from the animal and prepare them for cell culture in a multiwell plate. By diluting the mixture of cells so that each well receives either no cells or one cell, we can produce a single clone of...
Untreated THP-1 and THP-1 (treated with 80 nM PMA overnight, replaced the culture medium with 100 ng/mL LPS for 6 hours with addition of 300 ng/mL Brefeldin A for the last 3 hours) cells were subjected to SDS PAGE followed by western blot with (Product # 17590-1-AP) (TNF Alpha ...
Recombinant protein - amino acids 1-69 of mature rat CCL4 查看免疫原 偶联物 Unconjugated 形式 Liquid 浓度 1 mg/mL 纯化类型 Protein A 保存液 cell culture media 内含物 0.1% sodium azide 保存条件 Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycle...
immune rabbits to produce polyclonal antibody in routine.The specificity of the antibody was verified by Western blotting,and in situ immune was anaylzed by in vitro linear intramolecular assay system.The location and distribution of ΦC31 integrase in C31 integrase expressed HEK293 cells were ...
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- 《American Journal of Physiology》 被引量: 347发表: 1994年 Heparin-releasable lipoprotein lipase activity is increased in cardiomyocytes after culture. cardiomyocytes was dependent on serum (16.3-fold activation) and was inhibited by high ionic strength (1 M NaCl) and by a polyclonal antibody ...
pAbs are a set of antibodies formed in the body by various B cells. They have the ability to recognize and attach to a variety of antigen epitopes. An immunogen is injected into an animal to create pAbs.83 Following a primary immune reaction to a single antigen, on the other hand, ani...