For the production of monoclonal antibodies (mAbs), mice were immunized intraperitoneally with hyphae. The resulting hybridoma cell culture supernatants were tested by an indirect immunolabeling procedure. For this purpose the hyphae were immobilized on silane-coated microscopic slides. The mAb 8A7 ...
A micro cell culture system was applied to diphtheria toxin and antitoxin titrations using a colour change of phenol red as an indicator of the toxin activity on the cells. One of the characteristics of the present method is to seed cell... MSNIM Kono - 《Journal of Biological Standardizatio...
Now imagine that, instead of collecting the pooled sera, we isolate all of the immune cells from the animal and prepare them for cell culture in a multiwell plate. By diluting the mixture of cells so that each well receives either no cells or one cell, we can produce a single clone of...
The cloned hybridomas were stable and secreted up to 40 mg/L of antibody into the culture supernatant. Phospholipase A2 specificity of the human monoclonal antibodies was confirmed by binding and inhibition ELISA and by Western blot analysis. Epitope mapping on phospholipase A2 was done with the...
Xiao Wang1, Tien L. Olson1,¶, Hetal Patel1,§, Petra Fromme2,3,4 & Kathryn F. Sykes1,4,|| Antibodies are essential for structural determinations and functional studies of membrane proteins, but antibody generation is limited by the availability of properly-folded and purif...
Antibodies are essential for structural determinations and functional studies of membrane proteins, but antibody generation is limited by the availability of properly-folded and purified antigen. We describe the first application of genetic immunization to a structurally diverse set of membrane proteins to...
Recombinant protein - amino acids 1-69 of mature rat CCL4 查看免疫原 偶联物 Unconjugated 形式 Liquid 浓度 1 mg/mL 纯化类型 Protein A 保存液 cell culture media 内含物 0.1% sodium azide 保存条件 Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycle...
coli and can be used to identify neurons and their processes in culture or in sections. The antibody works cleanly on appropriate lysates of cell and tissues. |Stable at 4°C. 靶标信息 PGP9.5 (Protein gene product 9.5, UCH-L1, PARK5) is a neuron specific protein, structurally and ...
Monoclonal antibodies produced by a hybridoma are homogeneous, expressing a single amino acid sequence. ▪ Hybridomas can be grown indefinitely in tissue culture or in the peritoneal cavities of syngeneic mice (for murine hybridomas). ▪ Hybridomas can be selected on the basis of the antigenic...
Western Blotting with Rabbit Polyclonal Antibodies SAMPLE PREPARATION For Protein Concentration Determination of Cell Culture Decant medium from 10 cm dish of adherent cells and rinse plate rapidly with phosphate-buffered saline (PBS). Aspirate excess PBS....