Primary cell culture is a valuable tool to utilize in parallel with in vivo studies in order to maximize our understanding of the mechanisms surrounding neurogenesis and central nervous system (CNS) regeneration and plasticity. The zebrafish is an important model for biomedical research and primary ...
Isolation and primary culture of human proximal tubule cells . Methods Mol Biol 466 : 19 – 24 .Vesey DA, Qi W, Chen X, Pollock CA, Johnson DW (2009). Isolation and primary culture of human proximal tubule cells. Methods Mol Biol 466: 19-24....
Culture of adherent cells typically takes place on a plastic surface, which may be treated with plasma to change the surface chemistry, for example, or coated with natural or synthetic ECM proteins, to allow the cells to adhere. The specifics are dependent on cell type being ...
Background: Adipose tissue (AT) is a rich source of mesenchymal stem cells (MSCs), however, there is no standardized protocol for stem cell isolation and culture. This leads to inconsistency of the results and limits the comparison of the data from different laboratories. Our aim was to ...
Primary brain cell isolation and culture(5)1.Cerebella were removed from 7-day-old mice and passed through Nitex nylon netting (80 μm pore size) into primary cell system containing 20% (v/v) fetal calf serum (FCS).
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sion was centrifuged at 130 x g for 8 min at 4°C and resus- pended in 10 ml of culture media. Magnetic Purification of ATII Cells from Crude Cell Suspensions The cells were incubated with biotinylated anti-CD-32 (0.65 ~g/million cells) and biotinylated anti-CD-45 (1.5 ~g/million ...
The invention belongs to the technical field of cell isolation and cultivation. In order to solve the problems that the chicken small intestinal epithelial cells are difficult to isolate and culture in vitro at present, the existing isolation process is troublesome and time-consuming, the obtained ...
Primary cell cultures of bovine colon epithelium: Isolation and cell culture of colonocytes 来自 Semantic Scholar 喜欢 0 阅读量: 89 作者:W Fllmann,S Weber,S Birkner 摘要: Epithelial cells from bovine colon were isolated by mechanical preparation combined with an enzymatic digestion from colon ...
The neonate rabbit was used in this experiment,and collagenase Ⅳ digestion was used to isolate and culture the IEC of rabbit.The rabbit IECs were purified by the way of phase difference digestion and adherence.And the IEC was identified with morphological observation and cyto-keratin detection,usi...