Mechanistically, enrichment of regulatory T cells (Treg) in the skin was detected after PD-L1 fusion protein treatment in Pmel-1 mice. Furthermore, Tregs abundance was also increased in both the spleen and circulation of Pmel-1 mice treated with PD-L1. These data indicate that PD-L1 protein...
of melanosomes from stage I to II. The transition from stage I melanosomes to stage II melanosomes involves an elongation of the vesicle, and the appearance within of distinct fibrillar structures. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity...
Reporter assays, quantitative-PCR, Northern, and Western analysis all suggested that MITF either regulates reporters or the endogenous genes within melanoma cells and melanocytes. Protein and mRNA levels of the three antigens were found to correlate in a panel of melanoma cell culture lines as well...
Analysis of Pmel17 processing in glycosylation-deficient mutant cells reveals that Pmel17 lacking the correct addition of sialic acid and galactose loses the ability to form fibrils. Furthermore, we show that addition of sialic acid affects the stability and sorting of Pmel17 and reduces ...
The aim of our study was to evaluate the role of AP complexes in sorting of Pmel17 using purified melanosomal fractions, enriched with stage I melanosomes, from SKMel28 amelanotic melanoma cells. Melanosomal fractions were obtained after sucrose gradient separation and then analyzed using mass ...
Despite the importance of sialic acid addition, no study has reported sialyltransferase activity or its role in the sorting of melanosome-specific proteins in various types of melanocytic cells. N-Glycans on Pmel17 contains a mixture of complex, hybrid, and mannose-rich carbohydrate chains that ...
Adoptively transferred Pmel-1 T cells display lytic capability in the periphery after priming by tumor growth.David, A. SchaerYongbiao, LiTaha, MerghoubGabrielle, A. RizzutoAmos, ShemeshAdam, D. CohenYanyun, LiFrancesca, AvogadriRicardo, ToledoCrow...
Pmel-1 TCR tetramer staining distinguishes the gene dosage of pmel-1 transgene in CD8+ T cells.Yun JiNatalie AbramsWei ZhuEddie SalinasZhiya YuDouglas C. PalmerParthav JailwalaZulmarie FrancoRahul RoychoudhuriEric Stahlberg
Spectral cytometry analysis performed at 7 and 14 days after ACT showed that following NX-1607, a larger fraction of circulating Pmel-1 cells had a central-memory phenotype and expressed high levels of Granzyme B. Interestingly, both in vivo treatments induced increased 4-1BB/CD137 expression ...
In Vivo Synergy of IL-21 Plus IL-2 in A Murine Transgenic pmel-1 Therapeutic Melanoma Tumor Model Results in Significant Anti-Tumor Immunity and Durable Long-Term Curative Effectdendritic cellsimmunotherapyserum-free mediumanti-interleukin-10