Naïve B16 specific Pmel-1 T cells are primed by tumor growth and infiltrate tumors after adoptive transfer into tumor bearing mice.David, A. SchaerYongbiao, LiTaha, MerghoubGabrielle, A. RizzutoAmos, ShemeshAdam, D. CohenYanyun, Li
doi: 10.3390/molecules26123529. PMID: 34207849; PMCID: PMC8273697. Long Name Premelanosome Protein Entrez Gene IDs 6490 (Human); 20431 (Mouse) Alternate Names D12S53EP1; Gp100; ME20; ME20M; ME20-M; melanocyte protein mel 17; Melanocyte protein Pmel 17; Melanocytes lineage-specific antigen...
(A–J) Western blot analysis of SDS-lysed total membranes derived from Mel220 cells stably co-expressing the functional PMEL variant D211A with various PMEL loss-of-function mutants. PMEL-specific antibodies HMB45 (A,B) and I51 (C–J) were used. GM130 serves as loading control in (E–...
Cryopreserved, freshly-isolated (i.e. non-stimulated) CD+ T cells obtained from each donor were also analysed in these same assays in order to determine the basal 'in situ' level and the impact of in vitro stimulation on the calculated frequencies of peptide-specific responder CD4+ T cells ...
This allele has thus been extensively used as a specific dominant-negative mutant.2, 56, 57 Adenoviral infection efficiency approached 100% in these cells, based on GFP signal (data not shown) and was shown to produce strong expression of wild-type or mutant MITF by Western blot (data not ...
Taken together, these data show a high frequency of Pmell7/gp100-specific T cells in autologous antitumor CTL clones derived from peripheral blood of a melanoma patient. 展开 关键词: CTL immunotherapy melanoma DOI: 10.1111/1523-1747.ep12298177 ...
Melanocyte lineage-specific antigens recognized by monoclonal antibodies NKI-beteb, HMB-50, and HMB-45 are encoded by a single cDNA. The recognized by monoclonal (MAb) NKI-beteb are among the best diagnostic markers for . MAb NKI-beteb reacts with throughout development and does not cros....
Immunoelectron microscopy analysis of the detergent-insoluble fractions shows that the melanosomal fibrils are densely labeled with antibodies specific to both the PKD (HMB50) and RPT (HMB45) domains but not to the N terminus (αPmel-N) (Fig. 2A). This indicates that both the PKD and RPT ...
Thus, the specific occurrence of grey hair on the ventral part of the body after NB-360 treatment was related to compound level and indicates that the observed depigmentation was dependent on NB-360 exposure in skin. Subsequently, the reversibility of hair depigmentation was investigated in female...
PD-L1 protein reduces melanocyte-specific T cell enrichment in the skin. (A) Melanocyte-reactive T cells were detected by immunostaining for the Vβ12 TCR subunit in Pmel-1 mice with PD-L1 protein or control with specific anti-VB12 antibodies and then quantified (Control group = 6 case...