Pmel-1 TCR tetramer staining distinguishes the gene dosage of pmel-1 transgene in CD8+ T cells.Yun JiNatalie AbramsWei ZhuEddie SalinasZhiya YuDouglas C. PalmerParthav JailwalaZulmarie FrancoRahul RoychoudhuriEric Stahlberg
Fig1. 在同源的黑色素瘤细胞中,采用CRISPitope法将CD8+T细胞表位融合到不同内源性基因产物C-末端 A:CRISPitope法示例图; B:不同CD8+ T细胞表位的通用供体质粒的模块示例图。Hgp100是CD8+pmel-1 T细胞高度识别的高亲和力表位,为了探讨ACT对黑色素瘤细胞的作用,作者选择hgp100-CD8+T细胞表位模型来进行实验。 C:...
The absence of PD-1 rendered mice resistant to viral infection and suppressed tumor growth and tumor metastasis by reducing the antigen-recognition threshold and increasing the cytotoxic activity of CD8+ T cells. PD-1/PD-L1 pathway-targeted therapies are under development in multiple diseases, ...
.com Keywords: tyrosinase; gp 100/pmel17; melanoma; helper T cells; ELISPOT; vaccine The recent understanding of the molecular basis of T cell recogni- tion of human tumours has allowed for the development of vaccine trials targeting the induction of antigen-specific, tumour-reactive CD8+ CTL....
MLANA/MART1 (hereafter referred to as MLANA) was cloned by two separate groups using melanoma reactive cytotoxic T lymphocytes to screen a cDNA library derived from melanoma cells.27, 28 MLANA expression is restricted to melanocytes, melanomas, and retinal pigment epithelium.27 Its function remains...
IL-15 enhances the in vivo antitumor activity of tumor-reactive CD8+ T cells. T cells by using a T cell receptor transgenic mouse (pmel-1) whose CD8(+) T cells recognize an epitope derived from the self/melanoma antigen gp100... CA Klebanoff,SE Finkelstein,DR Surman,... - 《Proc Nat...
In both SKCM and SKCM-metastasis patients, PMEL expression is negatively correlated with the infiltration cells of CD8+ T cells, macrophages, and neutrophils. Programmed cell-death protein 1 just showed response rates ranging from 20% to 40% in patients with melanoma, so it is cr...