The presence of plasmids decreases the C and D periods, mean cell sizes, and initiation masses for chromosome replication by 14, 24, 38, and 18%, respectively, relative to corresponding values for plasmid-free host cells. Plasmid copy number has a negligible influence on these parameters, ...
The presence of plasmids decreases the C and D periods, mean cell sizes, and initiation masses for chromosome replication by 14, 24, 38, and 18%, respectively, relative to corresponding values for plasmid-free host cells...
A Chemical Inhibitor of Cell Growth Reduces Cell Size in Bacillus subtilis Bacteriaexhibit complex responses to biologically active smallmolecules. These responses include reductions in transcriptional andtranslational efficiency, alterations in metabolic flux, and in somecases, dramatic changes in growth and...
Localization of a mosquito-larval toxin of Bacillus sphaericus 1593 Cell-free wall, membrane, and cytoplasmic fractions were prepared from Bacillus sphaericus 1593, which exhibited toxic activity against larvae of the mosqu... PS Myers,AA Yousten - 《Applied & Environmental Microbiology》 被引量: ...
patterns of the miniP1 plasmid pZC176, the miniNR1 plasmid pRR933, and the high-copy miniNR1 derivative pRR942 were examined during the Escherichia coli cell division cycle and compared to the cycle-specific replication pattern of a minichromosome and the cycle nonspecific pattern of pBR322. ...
Retention of platelets by cellophane membranes within a specially designed test-cell was significantly increased when heparin was added to citrated whole blood.3. Though aspirin blocks the release reaction with and without heparin, it does... C. S. Thomson,CD Forbes,C.R.M. Prentice - 《Clin...
摘要: λ phage with amber mutations in the N gene can persist indefinitely as an independent replicating unit, a plasmid, in the cytoplasm of host E. coli cells. This newly discovered mode of λ phage lysogeny may shed light on the evolution of plasmids and temperate phage....
The possible changes of inflammatory factors, glial cell activation and pain sensitivity after pUDK-HGF administration were investigated by ELISA, western blot and Von Frey tests, respectively. Results In behavioural assays, we found that a single intramuscular or intrathecal injection of pUDK-HGF ...
The chloroform-phenol lysis method was the most efficient and reliable method of preparing plasmid DNA for plasmid profile analysis. The success of this method may be largely attributable to two factors: (1) the efficacy of cell lysis was independent of either an alkaline agent or of heat; (...
Mutants of avian sarcoma virus which lack a functional DNA polymerase were found to be nonselective in the incorporation of host cell tRNA's into virus par... GG Peters,J Hu - 《Journal of Virology》 被引量: 146发表: 1980年 Structure of the HIV-1 RNA packaging signal The 5' leader of...