This discovery could aid in the development of more efficient nonviral vectors for gene transfer to SMCs.doi:10.1038/gt.2008.83A M MillerD A DeanGene TherapyMiller AM, Dean DA. Cell-specific nuclear import of plasmid DNA in smooth muscle requires tissue-specific transcription factors and DNA ...
Cell-specific nuclear import of plasmid DNA One factor limiting the success of non-viral gene therapy vectors is the relative inability to target genes specifically to a desired cell type. To address... J Vacik,BS Dean,WE Zimmer,... - 《Gene Therapy》 被引量: 400发表: 1999年 cell-...
in 1944 Oswald Avery demonstrated in an experiment that acquisition of pathogenic properties is due to the transfer of cell-free genetic material "of the desoxyribose type" (DNA) [12]. Over the
4]. Elements of the present synthesis were presented then [11], e.g. that sex began even before the nuclear envelope, i.e. in a prekaryote phase of evolution (see Fig.1) and the dominant selective
Oestrogen receptor α AF-1 and AF-2 domains have cell population-specific functions in the mammary epithelium Oestrogen receptors α (ERα) are expressed in a subset of mammary epithelial cells. Here, the authors identify cells with low-ERα protein levels and show that distinct cell population...
In addition, co-expression of both Notch-ICD and Importin-α3 displays synergistic effects on cell proliferation. Taken together, our results suggest that Importin-α3 mediated nuclear import of Notch-ICD may play important role in regulation of Notch signaling. 展开 ...
Therefore, exon-II-containing MBP isoforms play a specific regulatory role not only in the early regulation of the myelin machinery, but in a more general context, also in (embryonic) cell proliferation. 2. Materials and methods 2.1. Cell culture 2.1.1. Primary oligodendrocytes Primary ...
As shown in Figure 1F, sgUSP10 led to increased K63-linked polyubiquitination by using an anti-K63 Ub-specific antibody. Next, we measured the effects of USP10 on PTEN Ub levels in the cell-free setting. The results showed that the addition of USP10 significantly reduced the ubiquitination...
The nuclear matrix stabilizes primed-specific genes in human pluripotent stem cells Ma et al. show that heterogeneous nuclear ribonucleoprotein U promotes the primed state in human pluripotent stem cells by interacting with nuclear matrix protein, Matrin-3, and regulating primed-specific genes. ...
The essential role of RNA and DNA in many physio-pathologic conditions justifies the intensive studies aimed to disclose the molecular basis of the interactions between nucleic acids and their modulators [1,2]. Nowadays, the modulation of specific genes is considered a well-established strategy to ...