Plasmid curing in Staphylococcus aureus by antibiotics affecting the bacterial cell walldoi:10.1111/j.1574-6968.1988.tb02759.xStaphylococcus aureusBActerial cell wallPlasmidAntibioticS. Abdalla-GalalINSERM, U. 65, Faculté de Médecine, Abune Kennedy, F-30000 Nîmes, FranceM. Ramuz...
During the plasmid purification process, large amounts of endotoxins are released from lysed bacteria, which can negatively impact cell growth, cell viability, transfection efficiency, protein expression, and even mimic sepsis in humans. Thermo Fisher offers purification kits that span necessary p...
The Enrichment of Plasmid Dnas, in Bacterial Cell Lysates, Using an Alkaline-pH Procedure that Does Not Permanently Denature Them.The Enrichment of Plasmid Dnas, in Bacterial Cell Lysates, Using an Alkaline-pH Procedure that Does Not Permanently Denature Them.Linkagehelicity...
内毒素,又称脂多糖或LPS,是大肠杆菌等革兰氏阴性菌细胞膜的组成部分,如大肠杆菌(参见"Bacterial cell wall")。内毒素在质粒裂解纯化过程中释放出来,并显著降低内毒素敏感细胞株的转染效率。此外,内毒素与DNA竞争“自由”转染试剂,影响转染实验中质粒DNA的摄取。内毒素也诱导巨噬细胞和B细胞等免疫细胞发生非特异性...
In contrast, microfluidics can precisely control a bacterial microenvironment under flow conditions, allowing long-term chemostat cultivation (Li et al., 2014a; B. Li et al., 2018). In combination with time-lapse imaging, real time monitoring of cell growth at the single cell level can be ...
1976年，J R Bedbrook等在Cell发表文章：《Recombination between bacterial plasmids leading to the formation of plasmid multimers》，他们发现pMB9质粒DNA在rec+大肠杆菌中呈现出各种不同的大小，更大形式的pMB9质粒DNA是最小的MB9质粒多聚体。这种质粒多聚体的形成是通过两个质粒上的同源区域发生同源重组形成的...
Visualization of Mismatch Repair in Bacterial Cells We determined the localizations of mismatch repair proteins in living Bacillus subtilis cells. MutS-GFP colocalized with the chromosome in all cells and fo... BT Smith,AD Grossman,GC Walker - 《Molecular Cell》 被引量: 153发表: 2001年 Visualiza...
1976年,J R Bedbrook等在Cell发表文章:《Recombination between bacterial plasmids leading to the formation of plasmid multimers》,他们发现pMB9质粒DNA在rec+大肠杆菌中呈现出各种不同的大小,更大形式的pMB9质粒DNA是最小的MB9质粒多聚体。这种质粒多聚体的形成是通过两个质粒上的同源区域发生同源重组形成的,而...
美['plæzmɪd] 英['plæzmɪd] n.〔遗〕质粒;质体 网络细菌质粒;质粒载体;细菌质体 复数:plasmids 英汉 英英 网络释义 n. 1. 〔遗〕质粒 2. 质体
1976年,J R Bedbrook等在Cell发表文章:《Recombination between bacterial plasmids leading to the formation of plasmid multimers》,他们发现pMB9质粒DNA在rec+大肠杆菌中呈现出各种不同的大小,更大形式的pMB9质粒DNA是最小的MB9质粒多聚体。这种质粒多聚体的形成是通过两个质粒上的同源区域发生同源重组形成的,而...