How to Extract Plasmid DNA 是在优酷播出的教育高清视频,于2014-03-05 13:49:02上线。视频内容简介:How to Extract Plasmid DNA
A comparison of different plasmid segregation systems reveals (i) how unrelated filament-forming and DNA-binding proteins have been adopted and modified to create a range of simple DNA segregating complexes and (ii) how subtle changes in the few components of these DNA segregation machines has led...
Unintentional Manipulation of Plasmid Copy Number As mentioned above, the plasmid insert can also influence copy number. For example, a high-copy pUC plasmid may replicate at medium or low copy numbers when ligated to very large DNA inserts, resulting in lower plasmid yields than expected. This...
However, colonies made up of cells that accept a plasmid with the DNA insertion remain white, because the DNA that was inserted into the cloning vector at the MCS disrupts the lacZ gene making it impossible for the cell to create a functional enzyme to metabolize x-gal. In other words, ...
The preferred method is through lentiviral transduction as this will confidently integrate the DNA into the host cell and stably express the gene of interest. Method 2: Plasmid Transfection The other method is through plasmid transfection, which although it has a lower success rate, may work for ...
Polymerase chain reaction (PCR) is a laboratory procedure that can create replicas of DNA. Explore the three steps of this revolutionary process: denaturation, annealing, and extension. Related to this Question How does PCR work? Can PCR work with a single-stranded DNA template?
To re-engineer them for delivering human genes, scientists "clean out" the harmful parts of the virus, insert a human gene into the virus' genetic material and then inject the virus into the body. Another type of vector is a plasmid, a ring of bacterial DNA into which human genes can ...
A compromised detection of radiation-induced plasmid DNA fragments results in underestimation of calculated damage yields. Electrophoretic methods are easy and cheap, but they can only detect a part of the fragments, neglecting the shortest ones. These can be detected with atomic force microscopy, but...
which would then be the estimated size in kilobases of the DNA in one of the bands from your sample. Plasmids Step 1 Note that you may or may not need to use the instructions in this section. Agarose gel electrophoresis is often used to confirm that a plasmid contains a given insert. ...
Themainenzymesneededarerestrictionendonucleaseswhichbreakthesugar-phoshatebackboneofDNAmoleculesatprecisesites,andDNAligaseswhichareabletojointogetherthefragmentsofDNAthataregeneratedinthisway(Figure3.3).OnceapieceofDNAhasbeeninsertedintoaplasmiditthenhastobeintroducedintothebacterialhostbyatransformationprocess....