Transfect cells at 40–80% confluency. Too few cells can cause poor growth due to limited cell-to-cell contact, while too many cells can lead to a resistance in uptaking foreign nucleic acid. Most chemical transfection reagents also have optimal time windows between 5 and 30 minutes, dependin...
Transfection is when eukaryotic cells take up foreign DNA or RNA by non-viral means. Mammalian and many other eukaryotic cells do not naturally take up foreign nucleic acids, but scientists have discovered ways to introduce plasmid and other foreign DNA or RNA into eukaryotic cells. Much like th...
What impact do viruses have on prokaryotic and eukaryotic cells? Why is mitochondrial DNA important to phylogenetics? What is the purpose of DNA polymerase? What is the purpose of DNA ligase? What is the purpose of DNA helicase? What is the function of DNA ligase in recombinant technology?
Bacteria are unicellular i.e. made up of a single cell. They are prokaryotes and their cells are different from animal and plant cells. In general, the structure of bacteria can be studied as external and internal structures; Structure of a Bacterial Cell ...
Plasmid A plasmid is a ring-shaped piece of DNA that is found within bacterial cells. Plasmids replicate independently of the chromosomal DNA found in the nucleoid but are always copied into next generation cells. Plasmids often contain genes that give bacteria genetic advantages such as antibiotic...
Do prokaryotic cells have RNA? In eukaryotes, describe what genes each RNA polymerase transcribes. In E.coli DNA replication, what is the enzyme that adds nucleotides to the growing daughter strand? Is the RNA and DNA polymerase in prokaryotes and eukaryotes the same? If not what are the dif...
Insertplasmidin bacteria 4. How do you insert the plasmid inside the bacteria? What process do you use? (1/2 pt) Identify bacteria that has the plasmid 5. In the last lab you swabbed four plates of agar with Get Access
Gene therapy medicines have emerged as a transformative class of medicines, especially with recent breakthroughs like CAR-T cells, or gene editing approaches for sickle cell anemia.1 Recent advancements, as different as permanent editing of the genome or the transient delivery of messenger RNA (mRNA...
Does impetigo have bacterial virulence factors? How did EHEC acquire many of its virulence factors? What is the theory of virulence? What virulence factor increases white blood cells? What is the virulence of HIV? What is the difference between disease potency, virulence, and etiology?
Plasmid Replicates independently within bacterial cells. Plasmids have their own origins of replication ensuring their maintenance in the host. 9 Cosmid Utilizes bacteriophage cos sites for DNA packaging. The inclusion of cos sites allows cosmids to package DNA into phage particles, enhancing delivery ...