of islet paracrine effectors (somatostatin (SST, 400 nM), insulin (INS, 100 nM), forskolin (FSK, 2 µM), ϒ-aminobutyric acic GABA (400 nM), adrenaline (ADR, 5 µM), or glutamate (Glut, 1 mM), all present in the bath) at 1 or 10 mM glucose (Fig...
Using U2OS-FUCCI cells that mark different phases of cell cycle33, we found that cell death in shATR cells was not dependent on the cell cycle phase (Supplementary Fig. 3b). We found comparable range of 53BP1-GFP foci in ATR-inhibited cells and in control cells; moreover, the presence ...
2014). Antimicrobial-resistant Escherichia coli (plasmid-mediated colistin resistance mechanism, MCR-1) originating from overcrowding and high-intensity farming of pigs in China (Liu et al. 2016) can at present be found in countries far from China, including the USA (Hu et al. 2016; Skov and...
The inhibitors were present throughout the assay. The concentration of the solvent was kept equal in all samples in a given experiment and maintained below 0.1% to avoid toxic effects. For each condition, a triplicate set of 1 mL cultures wa...
plasmid PX45953(Addgene, 62988) containing an sgRNA sequence targeting rpl12 (encoding uL11) near the start codon of the open reading frame (ORF), and an asymmetric dsDNA template54, for homology directed repair of the gene, using lipofectamine 2000. After transfection (24 h), cells were...
Minicircle (MC) DNA lacks plasmid backbone sequences and correspondingly confers higher levels of sustained transgene expression upon delivery, accounting for its success in preclinical gene therapy models. In this study, we show for the first time that MC DNA also functions as a vaccine platform....
sapiens (RecQ2 and RecQ3) and it has probable role in DNA binding [23]. The exonuclease domain is only present in human RecQ3 at the N-terminal position. Both PfRecQ homologues also contain the two distinct domains such as helicase and RQC domains but the unusual HRDC domain which is ...
(BDNF) plasmid DNA into human umbilical cord blood-derived MSCs (hUCB-MSCs) with significant efficiency, and investigated the stem cell potentiality of engineered MSCs through their phenotypes, proliferative capacity, ability to differentiate into multiple lineages, and migration ability towards malignant...
Innate immune responses are evolutionarily conserved processes that provide crucial protection against invading organisms. Gene activation by potent NF-κB transcription factors is essential both in mammals and Drosophila during infection and stress chal
a, b Yeast BY4741 and YK44 cells transformed with a pYES2 plasmid and a pYES2 plasmid that harbored BjHMA4R were grown in liquid YPD medium supplemented with 30 μM CdCl2. The cells were incubated at 30 °C for 10 h and 21 h. Fifteen milliliters of yeast fluid was ...