For a plasmid to be useful as a recombinant DNA vector, it must have some essential features. What features are important for a plasmid that is used in recombinant DNA procedures? After transforming E.coli with the plasmids, how can we identify the cells that contain a chimeric plasmid (cont...
How is the structure of prokaryotic and eukaryotic cells related to how their DNA is replicated? What is DNA replication and its relation to PCR? Do prokaryotic cells have RNA? In eukaryotes, describe what genes each RNA polymerase transcribes. ...
have their pros and cons and should be selected based on the resources available, the type of cells to be transfected, and the nature of the nucleic acid cargo to be delivered. Regardless of the method you choose, recovering endotoxin-free plasmid is critical to the success of your plasmid ...
Horizontal transfer exists largely because some MGEs can move or be mobilized between microbial cells and carry other traits than the ones strictly essential for their replication (Frost et al., 2005). They encompass a wide set of different elements, such as transposable elements, plasmids and ...
Eventually, the culture will become dominated by existing plasmid-free cells, leading to low plasmid yield. Plasmid Copy Number Summarized Plasmid copy number can influence experiments, and while it is appealing to have high-copy numbers for your plasmids, there are some applications where low plasm...
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Step-by-Step Solution:Step 1: Define Recombination Recombination is the biological process that results in the exchange of genetic material between different DNA molecules, leading to the formation of new combi
You observe a bacterium with a spirochete morphology. What type of flagella does this microbe have? How do spirochete flagella differ from peritrichous flagella in location and how does it move the microbe? How many cells does a virus have? Is it a single celled organism?
Which antibodies will you then have if you are A+? What is the definition of a zoonotic pathogen? What is the study of microbiology? What are the spikes in a viral capsid composed of? What is a bacterial colony? Which plasmids are present in the bacterial cells?
Which plasmids are present in the bacterial cells? What is bacterial transformation? What kind of cells has viruses that can infect them? What kind of cells are involved in both types of acquired immunity? What are the viral effects on the cell? What are the two mechanisms by which a bacte...