Considering these limitations, the delivery of therapeutic genes to target cells upon direct in vivo administration of non- viral vectors, i.e., plasmids, is of great value for the development of gene therapy. However, the use of plasmids is plagued by poor transfer efficiency, intracellular ...
SpecificityPCNA (proliferating cell nuclear antigen), tested in human cells and zebrafish ReporterTagRFP Vector typeMammalian expression vector PromoterConstitutive CMV IE Codon usageMammalian SelectionKan/Neo SequenceWith the Cell Cycle-Chromobody plasmid you receive the sequence information of the Alpaca an...
Specificity PCNA (proliferating cell nuclear antigen), tested in human cells and zebrafish Reporter TagRFP Vector type Mammalian expression vector Promoter Constitutive CMV IE Codon usage Mammalian Selection Kan/Neo Sequence With the Cell Cycle-Chromobody plasmid you receive the sequence information of the...
Construction and expression of the PrM E gene of dengue 2 virus 43 strain in mammalian cells; 我国登革2型病毒43株PrM-E基因的构建及其在哺乳动物细胞中的表达 更多例句>> 补充资料:动物细胞 分子式:CAS号:性质:来自动物体的细胞。体外培养的动物细胞可分为原代细胞和传代细胞。原代细胞是指从机体取出后...
SequenceWith the Cell Cycle-Chromobody plasmid you receive the sequence information of the Alpaca antibody to PCNA fused to TagRFP, as well as the full vector sequence. TransfectionTransfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipo...
A System for Stable Expression of Short Interfering RNAs in Mammalian Cells Mammalian genetic approaches to study gene function have been hampered by the lack of tools to generate stable loss-of-function phenotypes efficiently. We ... Brummelkamp,T. R. - 《Science》 被引量: 1.3万发表: 2002...
Overproduction of the poly(ADP-ribose) polymerase DNA-binding domain blocks alkylation-induced DNA repair synthesis in mammalian cells. in CV-1 monkey cells or in human fibroblasts to study their DNA-binding properties, the trans-dominant inhibition of resident PARP activity, and the cons... M,...
The DHFR cDNA, along with its promoter (MMTV-LTR) for plasmid amplification and the hygromycin B coding sequence, along with its TK promoter for selection in mammalian cells, are shown. AP(R): Start: 3965 End: 4828 IgG1 non genomic region: Start: 7234 End: 8214 VDJ of IGFR1 of 11...
All the elements required for replication and selection of the plasmid inE. coliand gene expression in mammalian cells are completely devoid of CpG dinucleotides. Furthermore, all Dam methylation sites (GATC) have been removed to prevent prokaryotic methylation. ...
In the current study, the eukaryotic expression plasmid pcDNA-nef was constructed by inserting a full-length nef gene into pcDNA3.1(+), and an in vitro transfection experiment suggested that the nef gene could be expressed successfully in mammalian cells. BALB/c mice were immunized with HIV-1 ...