Plasmids are replicating nonchromosomal (episomal) DNA molecules in prokaryotes and simple eukaryotes. Similar genetic elements in higher eukaryotes are normally exclusively referred to as episomes. Extra bits of DNA can be inserted into plasmids for piggy-back clone-wise replication – this procedure ...
AlthoughTi plasmidsare normally transferred fromAgrobacteriumto plant cells, the Ti plasmid is capable of entering the cells of other eukaryotes, at least in the laboratory. Yeast, somefilamentous fungi, and the cultivated mushroomAgaricushave all successfully received the Ti plasmid by conjugation from...
Note that the term “mitochondrial biogenesis” inside of the large category “translation” was based on KEGG categorization consisting of both prokaryotes and eukaryotes. The readers should thus treat these proteins here as translation factors. Moreover, genes involved in biosynthesis of surfactin and...
metagenomes, such as pBI143, a cryptic plasmid that was present in 52% of the gut metagenomes in our dataset, which we investigated in depth elsewhere44. However, the predicted plasmids were much more prevalent across human populations in general (Fig.3b); 63.1% of the predicted plasmids we...
Ch 16. Eukaryotes Ch 17. Virus Structure & Life Cycle Ch 18. Prokaryotic Cells Ch 19. Cell Biology & Cell Cycle Ch 20. Nerve, Muscle & Specialized Cell... Ch 21. Endocrine System: Hormones & Mechanisms... Ch 22. Nervous System: Structure, Function &... Ch 23. Circulatory System Ove...
Effective expression of small interfering RNA in human cells In many eukaryotes, expression of nuclear-encoded mRNA can be strongly inhibited by the presence of a double-stranded RNA (dsRNA) corresponding to exon seq... CP Paul,PD Good,I Winer,... - 《Nature Biotechnology》 被引量: 2588发表...
1. Determine the plasmid type and observe the location and quantity of Ori. Only one Ori plasmid may be prokaryotic cloning and expression plasmid. Two Ori plasmids may be shuttle plasmids that can replicate in both prokaryotes and eukaryotes. ...
The results revealed some features of homologous recombination reactions occurring in plant cells which cannot be accommodated by mechanisms postulated for similar reactions in animal system and in lower eukaryotes. 展开 关键词: Homologous recombination Protoplast transformation β-Glucuronidase Maize ...
Possible role of Escherichia coli penicillin-binding protein 6 in stabilization of stationary-phase peptidoglycan. Plasmids for high-level expression of penicillin-binding protein 6 (PBP6) were constructed, giving rise to overproduction of PBP6 under the control of the ... M P,VD Linden,D Haan,...
The discovery of these and many other regulatory systems during the heyday of plasmid biology in the 1980s and 1990s revealed the important role of regulatory RNAs long before their ubiquity was recognised in eukaryotes. They seemed to share a simple mechanism of action, exerting their effects th...