The gToW plasmid is present in low plasmid copy numbers in SC +Leu medium but in high copy numbers in SC-Leu medium. The tagged but not the native constructs are toxic when present in high copy numbers. (C) Micro-cultivation growth curves for the PDE2 constructs. (D) Schematic ...
The ectopic or heterochronic expression of a master regulatory gene such as Ndt80 in an unreduced plant cell, or before meiosis, could cause apomeiosis in a sexual plant. However, no homologs of Ndt80 have yet been reported in higher eukaryotes. View article Genetics of gametophyte biogenesis ...
In eukaryotes, the initiation of gene transcription requires RNA polymerase II, general transcription factors (TFIIA, TFIIB, TFIID, TFIIE, TFIIF, and TFIIH), DNA-binding transcription factors and transcription coactivators. The mediator complex is generally considered a transcription coactivator that forms...
Malaria-causing parasites of thePlasmodiumgenus undergo multiple developmental phases in the human and the mosquito hosts, regulated by various post-translational modifications. While ubiquitination by multi-component E3 ligases is key to regulate a wide range of cellular processes in eukaryotes, little i...
[56], and zeta toxins are associated with cell death [57]. The Plasmid 3 contains two CDSs homologous to type II toxin-antitoxin system of the PemK/MazF family that is involved in bacterial cell regulatory systems [58]. The other group of mobile genetic elements is transposable elements,L...
Histone wrapping of DNA into nucleosomes almost certainly evolved in the Archaea, and predates Eukaryotes. In Eukaryotes, nucleosome positioning plays a central role in regulating gene expression and is directed by primary sequence motifs that together form a nucleosome positioning code. The experiments...
petrii has 1,825 CDSs that are not present in either B. bronchiseptica or B. avium. A large number of these B. petrii-unique CDSs (1,157 CDSs and 188 genes encoding transposases) are located on mobile elements and encode accessory metabolic functions (Table 2). Figure 2 B. petrii ...
Nap1 is a conserved protein in eukaryotes involved in diverse physiological processes, such as nucleosome assembly, histone shuttling between the nucleus and cytoplasm, transcriptional regulation, and the cell cycle. Here, we identified Nap1 and characterized its roles in fungal development and ...
Histone post-translational modifications (HPTMs), including acetylation, phosphorylation, ubiquitination and 2-hydroxyisobutyrylation, play a crucial role in many cellular processes in eukaryotes, including secondary metabolite biosynthesis and chromatin regulation [6]. Among them, 2-hydroxyisobutyrylation is...
sis, respectively.174,175 The extent of conservation of this pathway In these images, the single-membrane bound vesicles released in eukaryotes was first revealed when various sequencing projects into the vacuole by fusion with autophagosomes (the autophagic were completed176 and it became evident ...