Other methods and techniques for depositing dry or semi-solid chemical compositions are known in the art and are not part of the principle invention. The reader should give the terms solid and immobilized form their broadest interpretation. H. Comparison of the Present Invention with Conventional ...
The details are best investigated for the multicopy plasmid ColE1, but the principle of using antisense RNA also applies to single-copy plasmids. Initiation of ColE1 replication starts with the transcription of an RNA molecule of 555 bases that can act as a primer for DNA synthesis. This pre...
This protocol presents a new method to purify plasmid DNA using temperature-triggered precipitation. The principle is based on the specific DNA-binding affinity of a bacterial metalloregulatory (MerR) protein to its cognate DNA sequence and the temperature responsiveness of elastin-like protein (ELP)...
Principle QIAprep 2.0 Spin Columns contain a unique silica membrane that binds up to 20 µg DNA in the presence of a high concentration of chaotropic salt, and allows elution in a small volume of low-salt buffer. QIAprep membrane technology eliminates time consuming phenol-chloroform ...
F. Transcription-driven supercoiling of DNA: direct biochemical evidence from in vitro studies. Cell 56, 111–118 (1989). Article CAS PubMed Google Scholar Dorman, C. J. & Dorman, M. J. DNA supercoiling is a fundamental regulatory principle in the control of bacterial gene expression. ...
The underlying principle for titration rests on the notion that iterons could inhibit DNA replication by tying up Rep initiators in nucleoprotein complexes that are nonproductive for replication initiation. A straightforward prediction of the titration model is that increasing the concentration of the ...
In principle there could be several different modes of integration, e.g. depending on the type of invertron or on the host itself. It could also be that secondary recombination events obscure the primary structure of integration sites, leading to false inferences on the mechanism of integration....
• Fast isolation of high purity plasmid DNA suitable for all conventional molecular biology procedures, including:• FastDigest or conventional restriction digestion• Automated fluorescent and radioactive sequencing• PCR• In vitro transcription• TransformationPrincipleA bacterial culture is ...
PrincipleQIAGEN Plasmid Plus Kit进行大规模质粒纯化,方便快捷。独特设计的QIAGEN Plasmid Plus纯化柱和创新的结合试剂,使其只需简单的结合–洗涤–洗脱过程。QIAGEN Plasmid Plus Kit可在20分钟内实现快速大规模的质粒制备。无需乙醇沉淀可直接用于下游各种应用。该质粒DNA适用于要求小体积量的应用。使用LyseBlue试剂,产量...
anatipestifer tonB1 gene was first used to proof-of-principle. The tonB1 gene was amplified and cloned into all three vectors, as described in the Materials and Methods section. Then, the three recombinant plasmids were introduced into the RA ATCC11845 ∆tonB1 strain. The transcription ...