Reagent Kits, DiagnosticPlasmidsTo isolate plasmid DNA from bacteria using a commercial plasmid miniprep kit (if interested, compare this protocol with Isolation of plasmid DNA from bacteria).doi:10.1016/B978-0-12-418687-3.00002-1Laura KoontzMethods in Enzymology...
How do scientists isolate ribosomal RNA? How does Z-DNA form? How does DNA ligase work? During translation in prokaryotes, what does the formation of the initiation complex require? How does retrovirus plasmid work? How are enzymes involved in DNA replication?
More advanced testing and surveillance methods, such as plasmid profiling and chromosomal fingerprinting, can also be used. So-called “genetic fingerprinting” of the bacterial isolate, using pulsed-field gel electrophoresis (PFGE), is a molecular technique that can help to characterizeShigellaisolates...
The leading strand only needs one primer for DNA polymerase to begin, but the lagging strand needs multiple primers, and DNA polymerase adds multiple DNA pieces between them, known as Okazaki fragments. The final step is termination once DNA polymerase h...
isolate was resistant to at least three antibiotics of different classes as declared by authors. Also, the average of the antimicrobial-resistance rate of different reports in the same year was used. The X-axes show the year of isolation....
Hemi-methylated DNA, such as after a fresh round of replication, is also protected from digestion. The restriction endonuclease and modification methylase genes lie adjacent to each other on the host chromosomal or plasmid DNA and may be oriented transcriptionally in a convergent, divergent, or ...
Transgene copy loss (He et al., 2012; Kim et al., 2011) may be directly linked to chromosomal rearrangements as well as to the specific way the transgene plasmid and copies thereof have integrated into the genome, where a high number of repeats may enhance the likelihood of rearrangements ...
be tested for susceptibility to an organism before it's used. And that's because there are mechanisms of aminoglycoside resistance that travel with carbapenemases on the same plasmid. So, on the same piece of DNA. And then when that piece of DNA moves, ...
revised form of Koch’s postulates known asmolecular Koch’s postulates. These are listed in the left column of Table 1. The premise for molecular Koch’s postulates is not in the ability to isolate a particular pathogen but rather to identify a gene that may cause the organism to be ...
The major “make-or-break” step during RNA extraction is phase separation (Figure 1). It requires dexterity and careful pipetting to transfer as much of the aqueous (RNA) phase as possible without disturbing the DNA-rich interphase.