19. A method for isolating plasmid DNA from bacteria by alkaline lysis, the method comprising: a) providing a bacterial suspension comprising bacteria having plasmid DNA; b) adding a modified P2 reagent directly to the bacterial suspension, wherein the P2 reagent comprises an alcohol to reduce SD...
our kits designed for low-endotoxin plasmid DNA isolation carefully remove these endotoxins, helping ensure the integrity of the isolated plasmid DNA. These kits offer a fast and easy method of plasmid purification, helping provide high purity and yield for sensitive downstream applications. Whether yo...
Method for the isolation of ccc plasmid DNA The present invention relates to the production of biomass for the isolation of ccc plasmid DNA comprising culturing a bacterial transformant in a bioreactor containing an antibiotic-free batch medium under batch-conditions and, at the e... T Schmidt,...
ArticlePubMedCASGoogle Scholar H.C. Birnboim and J. Doly, ‘A rapid alkaline extraction procedure for screening recombinant plasmid DNA’,Nucleic Acids Res.,7(1979), 1513–23. ArticlePubMedCASGoogle Scholar D.S. Holmes and M. Quigley, A rapid boiling method for the preparation of bacterial...
A novel method for rapid isolation of plasmid DNA A new disposable chromatographic column, pZ523, has been developed for separating plasmid DNA from bacterial chromosomal DNA. Use of pZ523 spun columns eli... PH Zervos,LM Morris,RJ Hellwig - 《Biotechniques》 被引量: 24发表: 1988年 ...
We developed a new simple high-throughput plasmid DNA extraction procedure, based on a modified alkaline lysis method, using only one 96-well microtiter gl... I Masayoshi,C Piero,N Sumiharu,... - 《Nucleic Acids Research》 被引量: 61发表: 1997年 On the stability of plasmid DNA vectors ...
Plasmid was first crudely prepared from bacterial cultures by a standard alkaline lysis method. After an alcohol precipitation, the nucleic acids were divided into two equal portions. One half was used for a standard purification method employing CsCl centrifucation. The other was dissolved in FPLC ...
Plasmids are double-stranded circular DNA molecules that have the property of self-replication, independent of chromosomal DNA. In bacteria, they carry gen... BIRNBOIM,H C - 《Methods in Enzymology》 被引量: 2225发表: 1983年 A nontoxic and versatile protein salting-out method for isolation of...
General Method for the Isolation of Plasmid Deoxyribonucleic Acid Plasmid deoxyribonucleic acid (DNA) ranging from 5 x 10(6) to 65 x 10(6) daltons may be isolated from chromosomal DNA by the preferential precipitation of ... PD Guerry,DJ Leblanc,S Falkow - 《Journal of Bacteriology》 被引量...
Endotoxin levels using different isolation methods.Efficient transfection in sensitive cell lines. Principle The QIAGEN Plasmid Plus Kits provide the most convenient method for large-scale plasmid preparation. The design and unique binding chemistry of the QIAGEN Plasmid ...