This exercise provides an introduction to the important techniques of microscale DNA isolation and analysis. Techniques such as these are central to modern molecular biology and provide an interesting contrast to the more traditional large-scale biochemical methodologies. The experiment itself will fit ...
Solutions of ELPs and NAs in each physiologically relevant fluid were prepared, and the workflow of the experiment is described in Fig. 3A. We note that the ELPs maintain their temperature-triggered phase behavior in artificial saliva and nasopharyngeal fluid. To our knowledge, LLPS of ELPs in...
Molecular biology techniques are no longer mysterious, and has become the main means of the contemporary system of biological research. This experiment, the integrated use of plasmid dna extraction, separation and purification of pcr amplification and dna connection with the conversion, receptive st ...
Infection experiment confirmed that the HIRRV CNPo2015 strain was virulent to flounder juveniles with a LD50value of 1.0 × 105.9TCID50/fish. In conclusion, we described the first isolation and characterization of a HIRRV from Japanese flounder in China. This will provide a candidate ...
Isolation of plasmid DNA and Agarose Gel Electrophoresis: Presence of plasmid DNA was confirmed by isolating plasmids from the pesticide tolerating bacteria by Miniprep method [9]. Pure cultures of all 05 isolates were grown overnight in 25 ml of Sterile Luria-Bertani bro...
Precise control of mammalian gene expression is facilitated through epigenetic mechanisms and nuclear organization. In particular, insulated chromosome structures are important for regulatory control, but the phenotypic consequences of their boundary dis
Furthermore, an artificial infection experiment was conducted to determine the virulence of the RGNNV-HL strain. Results from this study indicated that the virus isolated from adult hybrid Hulong grouper belonged to the RGNNV genotype. Findings from this study will provide a candidate material and ...
p) level allowed Boltzmann-weighted VCD spectra for both epimers of (–)-3 to be constructed. The final predicted spectrum was obtained by applying a 3:1 ratio to account for the experimentally analysed mixture of epimers. Numerical analysis was used to establish agreement between experiment and...
with antimicrobial activity against fish pathogenic bacteria were isolated from the soils of different regions in China. Streptomyces termitum N-15, which showed the strongest and most stable antibacterial activity, was selected for the fish disease control experiment. Then, we investigated the effect ...
For intra-cellular uptake experiment, RAW macrophages (200,000 cells) were seeded into 24 well TC plate containing glass coverslips. After 24hrs of culture, cells were treated with ENPs equivalent to 100μg, followed by incubation for different time points as indicated in figure legends. Cells ...