Bring the volume of the solution to 100 ml with deionised water and sterilize it by autoclaving it for 20 minutes at 15 psi.### Procedure**Isolation of Plasmid DNA**1. Pick up a colony of bacteria and inoculate it in a conical flask containing 100 ml autoclaved Luria broth media ...
A rapid and relatively simple procedure for purifying large quantities of plasmid DNA is described. Plasmid thus purified contains no detectable chromosomal DNA and little RNA or protein. The procedure combines alkaline denaturation and hydroxylapatite chromatography and utilizes an improved method of separ...
A new technique has been developed for the rapid isolation of covalently closed circular DNA molecules. The procedure is a selective extraction based on differences in the partitioning of covalently closed circular DNA molecules and nonc... Z Michael,Ginder Gordon D,F Gary - 《Nucleic Acids Resea...
A process is disclosed for the large scale isolation and purification of plasmid DNA from large scale microbial fermentations. All three forms of plasmid DNA; supercoil (form I), nicked or relaxed circle (form II), and linearized (form III), are individually isolatable using the disclosed pro...
In vitro insertional mutagenesis with a selectable DNA fragment. A new method for in vitro insertional mutagenesis of genes cloned in Escherichia coli is presented. This simple procedure combines the advantages of in vit... P Prentki,HM Krisch - 《Gene》 被引量: 3713发表: 1984年 The physical...
Isolation of DNA from filamentous fungi and separation into nuclear, mitochondrial, ribosomal, and plasmid components 来自 NCBI 喜欢 0 阅读量: 61 作者:RC Garber,OC Yoder 摘要: A general procedure for purifying and efficiently separating four types of DNA from filamentous fungi has been developed. ...
18. The apparatus of claim 16, wherein P2 and/or P3 is provided in solid or immobilized form. 19. A method for isolating plasmid DNA from bacteria by alkaline lysis, the method comprising: a) providing a bacterial suspension comprising bacteria having plasmid DNA; b) adding a modified P2...
申请(专利)号: US2007/016497 申请日期: 07/21/2007 公开/公告号: WO/2008/121121 申请(专利权)人: 发明人: X Jia 国省代号: WO 被引量: 8 摘要: The invention provides apparatus, reagents, and methods for rapidly isolating plasmid DNA from a bacterial alkaline lysate.收藏...
Procedure for ssM13 DNA Estimating DNA yield and quality Troubleshooting Related product information PureLink Kits Quant-iT Technology Introduction The PureLink HiPure Plasmid Purification Kits allow isolation of high yields of highly pure ssM13 (single-stranded M13) DNA from bac...
Isolation of Plasmid DNA from Butyrivibrio fibrisolvens. A procedure based on successive precipitation of cell lysates with sodium dodecyl sulfate-NaCl and polyethylene glycol 6000 was developed which allows the isolation of plasmid DNA from Butyrivibrio fibrisolvens. A survey of B. fibrisolve......