Restriction Maps Restriction enzymes can be used to construct maps of plasmid DNA Restriction enzymes can be used to construct maps of plasmid DNA Allows identification of restriction sites found in the sequence of the plasmid Allows identification of restriction sites found in the sequence of the pl...
Plasmids are small DNA molecules that enable bacteria to share beneficial traits, influencing microbial communities. However, their role within the human gut microbiome remains largely unknown. In this study, we investigate the gut microbiomes of 34 moth
Solutions and methods are disclosed for the effective, simple isolation of DNA from bacterial cells. High bioprocess recovery and product quality were primarily associated with the complete removal of total cellular RNA impurity. The process was demonstrated without the use of animal-derived RNase. ...
2.2. Vibrio Coralliilyticus Plasmid Isolation For large-scale plasmid isolation from V. coralliilyticus BAA-450, a 100 mL culture was incubated for 12 h, with plasmid DNA being released by alkaline lysis. The aqueous fraction was further extracted with phenol/chloroform (1:1) prior to ...
The shRNA is delivered into cells as DNA templates using a plasmid or a viral vector. The transcript, with a short hairpin loop ranging from fifty to one hundred nucleotides in length, is formed in the cell. Unlike siRNAs, shRNAs need to be processed within target cells before binding to ...