The present invention relates to novel, broad bacterial host range small plasmid deoxyribonucleic acid rings which serve as cloning vehicles for DNA fragments, particularly those separated from other plasmid rings or from chromosones, recombined with the small plasmid rings and to the processes for ...
E.coli.plasmid is frequently used as a cloning vector pBR322 Plasmid The main characteristics of pBR322 are: Restriction sites:BamH I, Hind III, Sal I, Pvu I, Pvu II, Pst I, EcoR I, Cla I Selectable marker:antibiotic resistance genes for ampicillin (ampR) and tetracycline (tetR) ...
plasmid cloning vector 专业医学词典 质粒克隆载体 与"plasmid cloning vector"相近的词条 ... plasmid analysis plasmid antibiotic-resistance plasmid plasmid chimaera plasmid chimera plasmid cloning use plasmid cloning vector plasmid compatibility plasmid construction plasmid copy number plasmid copy nunlber plasmid...
This chapter discusses the present generation of plasmid cloning vectors. It is not a comprehensive listing but rather a sampling of the most recent plasmids that illustrate the general trends in vector design. This is a massive tome on "cloning vectors" whose loose-leaf binder design allows new...
Plasmids are also found in higher organisms such as yeast and fungi. The 2 micron circle of yeast (discussed later) is a well-known example that has been modified for use as a cloning vector. Most plasmids are circular, made of DNA, and much smaller than chromosomes. The copy number is...
a strand or loop of DNA that exists independently of the chromosome in bacteria and yeast and that is capable of genetic replication: used in recombinant DNA procedures as a vehicle of gene transfer. [1952] Random House Kernerman Webster's College Dictionary, © 2010 K Dictionaries Ltd. Copyr...
often in the cytoplasm. Plasmids can be important in public health since some types possess genes for antibiotic resistance, and can be quickly transferred to different types of host cell, thus spreading resistance very rapidly. Plasmids form the basis of many cloning vectors used inGENETIC ENGINEER...
the present invention relates to a plasmid vector for cloning and expression of a protein in a microorganism.characterized in that it comprises at least the structural gene encoding the synthesis of the protein and the gene expression of element structure in a microorganism.and the promotion of th...
A plasmid cloning vector containing both transcriptional and translational regulatory sequences derived from the bacteriophage lambda genome was constructed to achieve high level expression of prokary
称为蓝白筛选法。lacpromoter MCSamprlacZ’pUC18 Ori氨苄青霉素/X-gal平板 H1DesignofPlasmidVectors ◎Ligationproducts◎Twinantibioticresistance◎Blue-whitescreening◎Multiplecloningsites◎Transcriptionofclonedinserts◎Expressionvectors 第二部分课件制作及讲解：胡婵娟 分子克隆技术 targetgene cloningvector ...