particularly for introducing modifications in gene coding regions. This method involves inducing frameshift mutations, altering intron splice junctions to encourage intron skipping, or creating large fragment deletions (Zhang et al.2022a). Various strategies have emerged to reduce target gene expression, i...
Hsp90 is an essential molecular chaperone responsible for the folding and activation of hundreds of ‘client’ proteins, including the glucocorticoid receptor (GR). Previously, we revealed that Hsp70 and Hsp90 remodel the conformation of GR to regulate l
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Hsp90 is an essential molecular chaperone responsible for the folding and activation of hundreds of ‘client’ proteins, including the glucocorticoid receptor (GR). Previously, we revealed that Hsp70 and Hsp90 remodel the conformation of GR to regulate l
In 1975, Milstein and Köhler revolutionized the medical world with the development of the hybridoma technique to produce monoclonal antibodies. Since then, monoclonal antibodies have entered almost every branch of biomedical research. Antibodies are n
The percentage of fusion inhibition was calculated with respect to the empty vector or non-treated control, respectively. The average of at least three independent experiments is shown. Error bars represent SEM. Full size image Initially, we investigated the role of clathrin and caveolae in DENV ...
it remained still largely unknown how the phage S-2L incorporates the base Z in its genome, especially as no gene corresponding to a DNA polymerase could be detected. This is in contrast with the situation in the phage φVC8, where a DNA polymerase of the family A has been identified16....
Would concerted efforts to construct a genome-wide deletion and/or overexpression library ofA. niger, similar to that existing forS. cerevisiae[95],N. crassa[96] andSchizosaccharomyces pombe[], be sufficiently helpful for the community to warrant the substantial investment of research funds and resou...
Truncated versions of MAGEA10 were generated by PCR and cloned into pQM-CMV-E2Tag vector (Icosagen; Tartu, Estonia). The list of primers used (Microsynth AG; Balgach, Switzerland) is shown in Table S1. To construct MAGEA10 deletion mutants fused in-frame with EGFP, the coding sequence ...
A pBI121-based construct, pBI-35S-PtCP5, was used to retrieve overexpressed PtCP5 transgenic Arabidopsis (OX-PtCP5). The expression of PtCP5 in independent transgenic plants was detected by qRT–PCR. The transgenic strains (OX-PtCP5: #3, #6, #8) with the highest PtCP5 expression levels...