The CaCl2-treated cells of A. aceti No. 1023 could also be rendered competent for uptake of plasmid DNA. In an attempt to produce an appropriate cloning vector for A. aceti, the restriction patterns of the cryptic plasmids, pTA5001 A (23.5 Kb) and pTA5001B (23 Kb), found in A. ...
redet同源重组系统介导的质粒载体快速构建 rapid construction of plasmid vector by redet mediated in vivo homologous recombination 星级: 6页 Red/ET同源重组系统介导的质粒载体快速构建,Red/ET同源重组系统介导的质粒载体快速构建 星级: 6页 redet同源重组系统介导的质粒载体快速构建 rapid construction of plasmi...
Construction of targeted plasmid vector pcDNA3.1-Egr.1p-p16 and its expression in pancreatic cancer JF305 cells induced by radiation AIM: To construct pcDNA3.1-Egr.1p-p16 recombinant plasmid and investigate the expression of p16 in pancreatic cancer JF305 cells induced by radiation and t... - ...
What is a plasmid in simple terms? A plasmid is a small circular DNA located separately from the chromosome of the host cell. It carries one or more genes, and it replicates independently. What is the difference between a plasmid and a vector? A plasmid is an extra-chromosomal DNA molecule...
After ligation of the construct into pET9c the plasmid was digested with BamHI and religated to remove the extraneous vector encoded BamHI site. The toxic nature of some lipoproteins when overexpressed influenced the Discussion The ability to produce recombinant acylated proteins offers some clear ...
Cloning of the gE gene of Pseudorabies virus Shanghai strains and construction of plasmid vector containing GFP gene 伪狂犬病病毒上海株gE基因克隆及其含GFP基因质粒载体的构建 www.ilib.cn 2. Construction of Plasmid with Green Fluorescent Protein Gene for Analysis of Yeasts in Yeast Wastewater Treatment...
Lambda YES: a multifunctional cDNA expression vector for the isolation of genes by complementation of yeast and Escherichia coli mutations. They also allow conversion from phage lambda to plasmid clones by using the cre-lox site-specific recombination system, referred to here as automatic sub... S...
Construction of a broad-host-range kanamycin-resistant plasmid vector - ScienceDirect 来自 Semantic Scholar 喜欢 0 阅读量: 52 作者: R Roychoudhury,A Lam 摘要: A new 10.2-kb plasmid, pIRL2, was constructed by using a 2140-bp DNA fragment carrying the Km r gene and Bam HI cohesive ends. ...
For industrial-scale production of xylitol, a plasmid-free Escherichia coli for arabitol-free xylitol production from corncob hemicellulosic hydrolysate has been constructed. Instead of being plasmid and inducer dependent, this strain relied on multiple-copy integration of xylose reductase (XR) genes ...
7.Constructing of Plant Expression Vector of rd22 from Tamarix Ramosissima and Its Functional Verification;柽柳rd22基因的载体构建及功能验证 8.Construction and Expression of the Gadd45a Gene and Its shRNAGadd45a基因真核表达载体及其RNA干扰载体的构建 9.Construction and Application of siRNA Plasmid for...