The invention relates to plasmid vectors containing an intact and active indicator gene at whose 3' end there is a multifunctional cloning region in which foreign DNA can be inserted while retaining the indicator function of the gene product, and to a method for the identification of genes ...
Lu, Q. (2004) Plasmid vectors for gene cloning and expression. In Plasmid Biology (Funnell, B.E. and Phillips, G.J., eds), pp. 545-566, ASM PressLu Q. Plasmid vectors for gene cloning and expression. In: Funnell BE, Phillips GJ, eds. Plasmid Biology. Washington, DC: American ...
4745062 Plasmid vectors for cloning and expression of a protein in a microorganism, comprising at least one promoter for expression of beta-glucosidase in yeasts; microorganisms containing these plasmids; a fermentation process and the enzymes obtained: Michel M Guerineau, Alain Raynal, Paris, France...
We have constructed cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp. All vectors contain the 760-bp oriT fragment from the IncP plasmid, RK2. Transfer functions need to be supplied in trans by the E. coli donor strain. We have incorporated into these...
for a replicative vector than to integrate a construct into the genome of cyanobacteria. Although both replicative and integrative vectors have specific characteristics, they share some important functional features, such as antibiotic resistance cassettes, mobilization elements, and cloning sites. ...
This chapter discusses the present generation of plasmid cloning vectors. It is not a comprehensive listing but rather a sampling of the most recent plasmids that illustrate the general trends in vector design. This is a massive tome on "cloning vectors" whose loose-leaf binder design allows new...
Versatile low-copy-number plasmid vectors for cloning in Escherichia coli. Small low-copy-number plasmid vectors were constructed by in vitro and in vivo recombinant DNA techniques. pLG338 and pLG339 are derived from pSC105, have ... NG Stoker,NF Fairweathe,BG Spratt - 《Gene》 被引量: ...
A Family of Compact Plasmid Vectors for DNA Immunization in Humans DNA immunization technology is based on the availability of adequate vectors for cloning and expression of heterologous immunoactive proteins in mammalian ... AM Herrera,EG Rodr?&Amp,#x0301,... - 《Biochemical & Biophysical Resear...
the present invention relates to a plasmid vector for cloning and expression of a protein in a microorganism.characterized in that it comprises at least the structural gene encoding the synthesis of the protein and the gene expression of element structure in a microorganism.and the promotion of th...
New and versatile cloning vectors with kanamycinresistance marker Gene (1987) R. Simon et al. Plasmid vectors for the genetic analysis and manipulation of Rhizobia and other Gramnegative bacteria Methods Enzymol. (1986) J. Tamaoka et al. Determination of DNA base composition by reversed-phase hig...