There are several situations where you want a high copy number plasmid, including: Protein expressionAlthough there is no significant advantage to using higher-copy-number plasmids over pBR322-based vectors in terms of protein production yields, a high-copy plasmid might be your first port of call...
aureus USA300 cells transformed with plasmids pErmCC and pLI50 (b), or pErmCC and pErmCC* (c). The percentage of cells expressing active ErmC was estimated by measuring the level of A2058 methylation every 10 generations for a total of ca. 40 generations. Values are the mean of three ...
introkitshave beendiscontinued.Note:Wedonotlinearizeourvectorsinhouse,butoffertheabovesuggestions astowhichsitestouse.Allthesesitesshouldlinearizetherespectiveplasmidinanareathatis inconsequentialtothosecomponentsthatfunctionpostintegrationintothemammaliangenome. ThemapsusedtodeterminethesesitesareofEMPTYvectors.Sinceeach...
In this unicellular system, replicators are sequence-defined genetic elements, too. S. cerevisiae origins support the extrachromosomal replication of plasmids and are therefore called autonomously replicating sequences (ARS) (Stinchcomb et al. 1979). They contain an ARS-consensus sequence (ACS or A ...