Watch complete video answer for “Why are cloning vectors bacteriophages sometimes preferred ov” of Biology Class 12th. Get FREE solutions to all questions from chapter BIOTECHNOLOGY AND ITS PRINCIPLES AND PROCESSES.
There are several situations where you want a high copy number plasmid, including: Protein expressionAlthough there is no significant advantage to using higher-copy-number plasmids over pBR322-based vectors in terms of protein production yields, a high-copy plasmid might be your first port of call...
After that, the OsWHY1-BD and OsTRX z-AD plasmids were co-transformed into yeast cells (Y2H Gold strain) and incubated for 3 days at 30 ℃ on a solid medium with- out leucine and tryptophan. The colonies were combined in 50 ul of sterile water before plating on a solid medium ...
In this unicellular system, replicators are sequence-defined genetic elements, too. S. cerevisiae origins support the extrachromosomal replication of plasmids and are therefore called autonomously replicating sequences (ARS) (Stinchcomb et al. 1979). They contain an ARS-consensus sequence (ACS or A ...
In the yeast assay, p53 variants are classified as functional, not functional, or partially functional according to the level of transcriptional activity quantified using the different reporter plasmids. Two high throughput p53 functional assays have recently been developed in human cancer cell lines. ...
coli clones harboring recombinant plasmids were validated by PCR, among which four clones were further sequenced. The recombinant plasmids were introduced into Agrobacterium tumefaciens (GV3101) via electroporation. The GV3101 was harvested when the OD600 reached ~1.0 grown with rifampicin (50 mg/L) ...