然后，MAPK13 磷酸化 TCF1，导致干细胞样 T 细胞增加。https://jitc.bmj.com/content/12/1/e008367 研究背景 01 在肿瘤微环境中，免疫细胞，特别是T细胞，进入耗竭或功能失调状态，细胞因子产生减少，抑制性受体表达持续增加，如程序性细胞死亡蛋白1（PD1）（由Pdcd1编码）和TIM3（由Havcr2编码），限制免疫治疗...
在肿瘤微环境中,免疫细胞,特别是T细胞,进入耗竭或功能失调状态,细胞因子产生减少,抑制性受体表达持续增加,如程序性细胞死亡蛋白1(PD1)(由Pdcd1编码)和TIM3(由Havcr2编码),限制免疫治疗,包括自体T细胞或嵌合抗原受体(CAR)T细胞的过继细胞治疗和...
本发明属于肿瘤免疫治疗技术领域,具体涉及终末耗竭T细胞群"TCF19+PD1+TIM3+CD8+"的鉴定及应用.本发明基于对终末耗竭T细胞群的单细胞测序数据进行分析,发现肿瘤组织内... 张常华,王付卉,董楠,... 被引量: 0发表: 2023年 终末耗竭T细胞群"TCF19+PD1+TIM3+CD8+"的鉴定及应用 本发明属于肿瘤免疫治疗技术领域,...
In the NAC group, the proportion of TCF1+CD8+ T cells was higher than that in the control group, as were the proportions of TCF1+PD1+CD8+ T cells (Fig. 4A, B), suggesting that NAC could induce Tprog.ex. differentiation. The proportion of PD1+Tim3+CD8+ T cells in the NAC group...
Furthermore, MACS-isolated CD8+T-cells from human-PBMC or murine-spleenocytes were repeatedly exposed to tumor-lysate and tumor-supernatant in presence of antigen-loaded DCs for 120h to obtain CD8+TTEX (PD1+TIM3+TCF1-CXCR5-IFN纬low) in-vitro. These CD8+TTEX were co-cultured with MCF7,...
Despite the increased PD1 levels, an improved PD1+CD28+ T-cell polyfunctionality was observed with the transition from periphery to tumor site, associated with lack of TIGIT, TIM-3 and LAG-3, but not with Ag-experienced-marker CD11a. Differently from CD28+ T cells, the increased PD1 levels...
T cells (O), effector PD-1+TCF-1−CD44+ TAG-specific CD8+ T cells (P), cytotoxic Granzyme B+ (GZMB) CD8+ T cells (Q), fresh effector PD-1+CD218a+ CD8+ T cells (R), better effector PD-1+CD218+Tim3high CD8+ T cells (S), and Ki67+ CD8+ T cells (T) (n = 3–10...
LAG-3, and TIM-3 [26]. Additionally, the expression of CXCR5, recognized as a distinctive marker of proliferative T cells following PD1 blockade [20], was most pronounced in PD1+CD28+T cell clones [27], thereby confirming a strong trend towards higher CXCR5 mRNA expression (Fig.1E). Whi...
3b). Immune checkpoint molecule PD1 and T cell immunoglobulin mucin family member 3 (TIM3) expression was upregulated on CD8+ T cells after co-culturing with THP-1 and Huh-7OE supernatant, but we found no different expression of PD1 or TIM3 in CD8+ T cells between co-culturing with ...
在肿瘤微环境中,免疫细胞,特别是T细胞,进入耗竭或功能失调状态,细胞因子产生减少,抑制性受体表达持续增加,如程序性细胞死亡蛋白1(PD1)(由Pdcd1编码)和TIM3(由Havcr2编码),限制免疫治疗,包括自体T细胞或嵌合抗原受体(CAR)T细胞的过继细胞治疗和抑制性受体的阻断, 这在临床癌症治疗中取得了显著的成功。