2. Mauro Poggio, et al. Suppression of Exosomal PD-L1Induces Systemic Anti-tumor Immunity and MemoryCell 177, 414–427, April 4, 2019. 3. Bo Shao, et al. Effectsof Tumor-Derived ExosomeProgrammed Death Ligand 1 on Tumor Immunity andClinical Applications. FrontCell Dev Biol. 2021; 9: 760...
01外泌体与肿瘤生长和侵袭相关 外泌体(exosome)是由活细胞分泌的直径约为40-160 nm的小囊泡,具有典型的脂质双分子层结构。包含有细胞的许多成分组成,包括DNA、RNA、氨基酸、蛋白质、代谢物、酶、脂质(胆固醇)和热休克蛋白,以及一些参与细胞间通讯的胞质和细胞表面信号蛋白等。胞外体膜富含跨膜蛋白(如CD81、CD63...
In this manuscript, we review the important roles of exosome-enriched PD-L1 (exo-PDL1) during WJMSCmediated therapy for acute graft-versus-host disease (aGvHD). We focus on exo-PD-L1's immunomodulation on T cell receptor (TCR) signaling in CD4+ T cells. In addition, we extend our ...
exosomes with anti-PD-L1 neutralizing antibody. H460 cell-derived and H1975 cell-derived exosomes with high PD-L1 levels significantly reversed the exosome-induced reduction of INF-γ production (Fig.3a), whereas A549 cell-derived exosomes with low PD-L1 levels did not. To confirm these findin...
[2]Xinyi Tu et al. PD-L1 (B7-H1) Competes with the RNA Exosome to Regulate the DNA Damage Response and Can Be Targeted to Sensitize to Radiation or Chemotherapy. Molecular Cell(2019). 参考资料: 1#Research decode...
(文献题目:Upregulation of exosome secretion from tumor- associated macrophages plays a key role in the suppression ofanti-tumorimmunity) 为了研究肿瘤相关巨噬细胞(TAM),作者首先诱导人源单核细胞成为巨噬细胞(Mφ),然后采用人黑色素瘤细胞WM9的培养上清液,模拟肿瘤微环境,使巨噬细胞转变成细长的纺锤状(=肿...
1.Effectsof Tumor-Derived Exosome Programmed Death Ligand 1 on Tumor Immunity andClinical Applications. Front Cell Dev Biol. 2021; 9: 760211. 公众号已建立“小药-生物资源对接服务平台”微信行业交流群以及读者交流群,扫描下方小编二维码加入,入行业群请主动告知姓名、工作...
作者发现对PD-L1药物不敏感的前列腺癌细胞虽然细胞表面PD-L1表达很低但合成的PD-L1并不少,只是这些肿瘤细胞把多数合成的PD-L1通过外泌体(exosome)秘密送到淋巴结而在免疫系统总部抑制免疫应答。如果用CRISPR技术敲除肿瘤细胞外泌体合成关键酶(Rab27或nSMNase)则免疫应答恢复。不仅如此,作者把Rab27或nSMNase敲除肿瘤...
4. Bo Shao, et al. Effectsof Tumor-Derived ExosomeProgrammed Death Ligand 1 on Tumor Immunity andClinical Applications. FrontCell Dev Biol. 2021; 9: 760211. 5. Dhouha Daassi , et al. The importance of exosomal PD-L1intumour immune evasion.Nat Rev Immunol. 2020 Apr;20(4):209-215. ...
The same amounts of exosome proteins from IFN-γ-treated and control cells were loaded (left). Quantification of the exosomal PD-L1 level determined by western blot analysis (right). d, Immunoblots for PD-L1 in the whole cell lysate or in the purified exosomes from control or IFN-γ-...