淋巴链式激活的免疫细胞(CAPRI) 是在CIK的基础上发展而来,CAPRI主要是利用患者自身的PBMC,加入CD3单抗和IL-2激活,并在体外将初始T细胞诱导为杀伤性效应细胞。CAPRI细胞治疗的临床效果优于CIK、LAK等细胞治疗,延长了转移性乳腺癌、非小细胞肺癌等多种肿瘤患者的生存时间。 3.体外受精治疗——PBMCs宫腔灌注 淋巴在很多...
six patients with PDDM 值得一提的是这个数据挖掘是韩国人做的哦,标题是:《Immunological link between periodontitis and type 2 diabetes deciphered by single‐cell RNA analysis》 首先是降维聚类分群和分组后看比例变化 如下所示: 看比例变化 然后是各个单细胞亚群在不同分组的各种差异分析和富集分析 如下所示...
wget https://cf.10xgenomics.com/samples/cell-atac/1.0.1/atac_v1_pbmc_10k/atac_v1_pbmc_10k_filtered_peak_bc_matrix.h5 wget https://cf.10xgenomics.com/samples/cell-atac/1.0.1/atac_v1_pbmc_10k/atac_v1_pbmc_10k_singlecell.csv wget https://cf.10xgenomics.com/samples/cell-atac/1.0.1...
大胆地定义细胞的类型吧。 [1]https://divingintogeneticsandgenomics.rbind.io/post/stacked-violin-plot-for-visualizing-single-cell-data-in-seurat/ [2]https://rpubs.com/DarrenVan/628853 [3]https://www.stemexpress.com/blogs/peripheral-blood-mononuclear-cells/...
是文章: Single cell transcriptomic landscape of diabetic foot ulcers.Nat Commun2022 ,它的第一层次降维聚类分群要复杂一点,一步到位了: 本质上也是读取文件即可,代码是: library(data.table) dir='GSE165816_RAW/'samples=list.files( dir ) sampleslibrary(data.table) ...
wget https://cf.10xgenomics.com/samples/cell-atac/1.0.1/atac_v1_pbmc_10k/atac_v1_pbmc_10k_filtered_peak_bc_matrix.h5 wget https://cf.10xgenomics.com/samples/cell-atac/1.0.1/atac_v1_pbmc_10k/atac_v1_pbmc_10k_singlecell.csv
[1] Jovic, Dragomirka et al. “Single-cell RNA sequencing technologies and applications: A brief overview.” Clinical and translational medicine vol. 12,3 (2022): e694. doi:10.1002/ctm2.694 [2] Geirsdottir, Laufey et al. “Cross-Species Single-Cell Analysis Reveals Divergence of the Prima...
Clustering metrics for single-cell RNA sequencing (scRNA-seq) of peripheral blood mononuclear cells (PBMCs) are used to assess the quality and reliability of the clustering results. These metrics help in evaluating the performance of different clustering algorithms and parameter settings. One commonly...
1. A single-cell map for the transcriptomic signatures of peripheral blood mononuclear cells in end stage renal disease 这是今年刚发的一篇文章,影响因子5.991。因为是做PBMC的,来学习一下分析思路和亚群注释的方法。 背景:终末期肾衰存在systemic inflammation和immune deficiency,但背后机制未知。
wget https://cf.10xgenomics.com/samples/cell-atac/1.0.1/atac_v1_pbmc_10k/atac_v1_pbmc_10k_filtered_peak_bc_matrix.h5 wget https://cf.10xgenomics.com/samples/cell-atac/1.0.1/atac_v1_pbmc_10k/atac_v1_pbmc_10k_singlecell.csv