长读长单分子测序技术(Long read single-molecule sequencing technology)又称第三代测序技术TGS(Third-Generation Sequencing),早在2004年,由美国太平洋生物科学公司Pacific Biosciences (PacBio) 创立的实时(SMRT)测序是最早被广泛使用的长读测序技术,SMRT测序产生的Reads可达到约200 kb。其提供了技术上的优势,以鉴定遗...
Overview of PacBio SMRT sequencing: principles, workflow, and applications PacBio's SMRT (single molecule real time) sequencing is one of the most commonly used third-generation sequencing technologie... A Benchmark Study on Error Assessment and Quality Control of CCS Reads Derived from the PacBi...
This article, published in the Annals of Laboratory Medicine, discusses the use of PacBio third-generation sequencing to identify a specific ABO gene promoter mutation, c.–35_–18del, which leads to weakened B antigen expression. The study describes two cases of patients with this...
With third-generation sequencing tools, researchers have been able to better impute complex traits… Learn more INCREASING THE THROUGHPUT OF FULL-LENGTH 16S AND RNA-SEQ WITH KINNEX KITS Discover the transformative power of Kinnex kits for advancing plant and animal research. Learn more Explore ...
Fan X,et al.SMOOTH-seq: single-cell genome sequencing of human cells on a third-generation sequencing platform.Genome Biol22,195 (2021). Adey A,et al.Rapid,low-input,low-bias construction of shotgun fragment libraries by high-density in vitro transposition.Genome Biol11,R119 (2010). ...
PacBio RS II测序仪是太平洋生物技术公司(Pacific Biosciences)基于单分子实时(SMRT)和纳米微孔应用的革命性的第三代测序(Third generation sequencing,TGS)平台,可以在一天内完成从样品制备到测序和读取序列的全过程。未来组是中国首家正式推出第三代测序服务的公司,在小基因组完成图、表观基因组、动植物基因组组装、...
2024年7月15日,中山大学附属第五医院生物信息学实验室的李淼新团队在Genome Biology发表了题为“Comprehensive and deep evaluation of structural variation detection pipelines with third-generation sequencing data”的研究论文,为我们解答了这个问题。他们使用来自PacBio(CLR、CCS)和Nanopore(ONT)平台的模拟和真实数据...
6. Fan X, et al. SMOOTH-seq: single-cell genome sequencing of human cells on a third-generation sequencing platform.Genome Biol22, 195 (2021). 7. Adey A, et al. Rapid, low-input, low-bias construction of shotgun fragment libraries by high-density in vitro transposition.Genome Biol 11...
6. Fan X, et al. SMOOTH-seq: single-cell genome sequencing of human cells on a third-generation sequencing platform. Genome Biol 22, 195 (2021). 7. Adey A, et al. Rapid, low-input, low-bias construction of shot...
Key words: Magnolia officinalis,genome,the third-generation sequencing technology,gene annotation, medicinal plant 隨着人类基因组计划的完成,基因组测序技术的不断发展和逐渐成熟,尤其以第三代测序技术发展为单分子实时测序,这加速了植物全基因组研究的进程。基因组大小是指某个物种单倍体基因组的全部DNA碱基对数,...