Explore HiFi sequencing systems Learn how to choose the right system for your needs. Learn more Talk with an expert If you have a question, need to check the status of an order, or are interested in purchasing an instrument, we're here to help. ...
Discover how highly accurate long-read sequencing, known as HiFi sequencing, is enabling scientific discoveries with data that is both accurate and complete.
To generate circular PacBio sequencing libraries, LILAP requires five steps (Fig.1c, “Methods”). First, Tn5 transposase mediates tagmentation, where adapter ligation and genomic DNA (gDNA) fragmentation are achieved simultaneously27,28. In contrast to linear adapters that are widely used in short...
SMRT(SingleMolecule,Real-TimeSequencing)技术和OxfordNanopore公司的Nanopore技术(Eid et al, 2009)。三代测序技术的主要特点是单分子实时测序、长读长, 但错误率偏高。下面从技术特点和测序原理两方面来介绍第三代测序技术。 1.3.1SMRT技术SMRT技术的核心是能够实现单个DNA分子的测序, 并且实时监控测序结果 ...
After a PacBio sequencing run, DeepConsensus is meant to be run on the subreads to create new corrected reads in FASTQ format that can take the place of the CCS/HiFi reads for downstream analyses. For variant-calling downstream For context, we are the team that created and maintains both ...
Mapping single molecule sequencing reads using basic local alignment with successive refinement (BLASR): application and theory. Mark J Chaisson; Glenn Tesler. (2012) SHRiMP: Accurate Mapping of Short Color-space Reads. Stephen M Rumble; Phil Lacroute; Adrian V. Dalca; Marc Fiume; Arend Sidow;...
Long-read sequencing, exemplified by PacBio, revolutionizes genomics, overcoming challenges like repetitive sequences. However, the high DNA requirement ( > 1 µg) is prohibitive for small organisms. We develop a low-input (100 ng), low-
Join us as we dive into what SBB sequencing is, how it works, and what its disruptive capabilities will mean for the future of research with short-read sequencing. The difference is in the chemistry In contrast to the major strides seen in long-read sequencing technology in recent years...
Explore HiFi sequencing systems Learn how to choose the right system for your needs. Learn more Talk with an expert If you have a question, need to check the status of an order, or are interested in purchasing an instrument, we're here to help....
How it works The reads are mapped to the reference sequence then variants are called. Learn more about SMRT Analysis and structural variant calling PacBio DevNet tools There are several community-supported tools for structural variant calling using SMRT sequencing data. They provide scientists with ea...