PacBio highly accurate long-read sequencing provides the most comprehensive view of genomes, transcriptomes, and epigenomes. Learn how SMRT sequencing powers genomic insights in humans, plants, animals and microbes.
“HiFi long read sequencing can now produce the most comprehensive variant dataset obtainable by a single technology in a single laboratory, allowing accurate calling of substitutions, indels, STRs and SVs.” –Kucuk et al., Genome Med, 2023 ...
even in hard-to-sequence regions of the genome. PacBio HiFi reads is the only long read sequencing technology that provide accuracy of 99.9%, on par with short reads and Sanger sequencing. With HiFi reads, you no longer have to compromise long read lengths for high accuracy sequencing to ad...
长读长测序最常用的方法是Oxford Nanopore Technologies(ONT)的纳米孔测序和PacBio的单分子实时(SMRT)测序。 冰岛deCODE Genetics公司的研究团队在Genome Biology发表了题为“A comparison of methods for detecting DNA methylation from long- read sequencing of human genomes”的文章,首次对长读长测序的CpG甲基化检测...
英文名:PacBio Single-Molecule Long-Read Sequencing Reveals Genes Tolerating Manganese Stress in Schima superba Saplings 杂志:Frontiers in Genetics 影响因子:4.599 研究背景 木荷(Schima superba) 是一种亚热带常绿乔木,被广泛用于森林防火和园艺。同时它是一种耐盐的植物,会在叶子中积累大量的锰。同时该树种生...
Pacific Biosciences公司近日以单分子环形一致性测序(CCS)为基础开发出一种方案,能够在Sequel测序平台上生成高度准确的长reads。这种方法有望克服短读长测序的读长不足以及长读长测序的准确性有限的问题。 这篇题为“Highly-accurate long-read sequencing improves variant detection and assembly of a human genome”的...
今天给大家介绍的这篇刚刚发表在bioRxiv杂志的文献 “Highly-accurate long-read sequencing improves variant detection and assembly of a human genome” 正是使用PacBio刚发布不久的Sequel System 6.0 结合13.5 kb文库,运用CCS(circular consensus sequencing)测序方式进行人类基因组的组装。实现了在PacBio Sequel平台上...
[1]Wenger A M , Peluso P , Rowell W J , et al. Accurate circular consensus long-read sequencing improves variant detection and assembly of a human genome[J]. Nature Biotechnology, 2019, 37(11). [2]PacBio.In precisionFDA Challenge,PacBio HiFi Reads Outperform Both Short Reads and Noisy ...
2.PacBio-RCIGM Long-Read Sequencing Study Aims to Identify Rare Diseases https://www.clinicalomics.com/topics/patient-care/rare-and-neglected-diseases/pacbio-rcigm-long-read-sequencing-study-aims-to-identify-rare-diseases...
"The speed and ease of use of the HiFi Prep Kit 96 and HiFi Plex Prep Kit 96 has made it possible for us to use long-read sequencing as a high-throughput genotyping tool." The new HiFi library prep kits are expected to be available for purchase in the ...